Dataset Information

Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection

ABSTRACT: Emerging evidence indicates that viral infection alters protein complex composition to induce both proviral and antiviral effects. However, global changes in protein complexes during infection remains understudied. Here, we employ thermal proximity coaggregation (TPCA) analysis to provide the first global and temporal characterization of changes in protein complexes during human cytomegalovirus (HCMV) infection. This dataset provides a resource to mine the alterations in protein complexes during HCMV infection. Furthermore, our subsequent experiments identified that these changes in protein complex composition and interactions functionally contribute to the progression of infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Human Cytomegalovirus (strain Ad169) (hhv-5) (human Herpesvirus 5)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Yutaka Hashimoto

LAB HEAD: Ileana M. Cristea

PROVIDER: PXD014747 | Pride | 2020-01-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	190201_CETSA_0hpi_2.msf	Msf
	190201_CETSA_0hpi_3.msf	Msf
	190201_CETSA_0hpi_3_Fr1.raw	Raw
	190201_CETSA_0hpi_3_Fr10.raw	Raw
	190201_CETSA_0hpi_3_Fr2.raw	Raw

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Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection.

Hashimoto Yutaka Y Sheng Xinlei X Murray-Nerger Laura A LA Cristea Ileana M IM

Nature communications 20200210 1

The co-evolution and co-existence of viral pathogens with their hosts for millions of years is reflected in dynamic virus-host protein-protein interactions (PPIs) that are intrinsic to the spread of infections. Here, we investigate the system-wide dynamics of protein complexes throughout infection with the herpesvirus, human cytomegalovirus (HCMV). Integrating thermal shift assays and mass spectrometry quantification with virology and microscopy, we monitor the temporal formation and dissociatio ...[more]

PMID: 32041945

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