Proteomics

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0

DMXL1 interaction proteomics from U2OS cells


ABSTRACT: To identify protein interactions with DMXL1, U2OS cells were engineered to either stably over-express DMXL1 tagged with mNeonGreen (mNG) or express HA-dTAG-tagged DMXL1 from the endogenous locus. Tagged DMXL1 was pulled-down by immunoprecipitation (IP) and IP samples were processed for TMT-MS analysis. As negative control, pull-down was also performed from parental U2OS cells. Each condition was performed in triplicate.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Chan Lee  

LAB HEAD: J. Wade

PROVIDER: PXD054967 | Pride | 2025-04-22

REPOSITORIES: Pride

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