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Quantitative proteome profiling in HEK293 to validate RNAi targets

ABSTRACT: The solute carrier (SLC) transporter superfamily are important coordinators of metabolic and cellular homeostasis. Despite their importance for cellular homeostasis, SLCs remain a largely understudied group of proteins regarding their protein interactome. Thus, we conducted a largescale interactome proteomics study to characterize the interactome of roughly 400 of the 450 superfamily members. Within this study, we validated selected SLC-protein interactions by different assays (e.g.: protein stability, transporter activity assays), in combination with RNA interference (RNAi). For a small subset of the targeted interactors, we used full protein profiling by quantitative mass spectrometry to confirm alleviation of the protein abundance upon RNAi. To validate RNAi downregulation of gene expression on protein level, we assessed protein abundance of ZPFL1, LTN1 and NFXL1 after siRNA treatment.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney Cell

SUBMITTER: Fabian Frommelt

LAB HEAD: Giulio Superti-Furga

PROVIDER: PXD055192 | Pride | 2025-03-03

REPOSITORIES: Pride

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			Action	DRS
	20240621_172405_20240621_RESOLUTE_full_proteome.sne	Other
	20240621_184330_20240621_RESOLUTE_full_proteome_Report.params	Other
	20240621_184330_20240621_RESOLUTE_full_proteome_Report.setup.txt	Txt
	20240621_184330_20240621_RESOLUTE_full_proteome_Report.tsv	Tabular
	2024_full_proteome_RNAi_DIA_protein_level.txt	Txt

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