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Determining the changes in the immunopeptidome in vivo following treatment with Papaverine

ABSTRACT: The aim of this project is to understand whether the alleviation of hypoxia in vivo leads to changes in the immunopeptidome. In this experiment, mouse colorectal CT26 cancer cells were subcutaneously transplanted into syngeneic (Balb/c) mice. Papaverine was administrated every 24h for 3 days before tumor was harvested ans immunopeptidomics experiment was then carried ou.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

SUBMITTER: Nicola Ternette

LAB HEAD: Nicola Ternette

PROVIDER: PXD055211 | Pride | 2024-11-04

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	HFX255_IPP436_HE_CT26PPV00_MHCI.mgf	Mgf
	HFX255_IPP436_HE_CT26PPV00_MHCI.raw	Raw
	HFX255_IPP436_HE_CT26PPV01_MHCI.mgf	Mgf
	HFX255_IPP436_HE_CT26PPV01_MHCI.raw	Raw
	HFX255_IPP436_HE_CT26PPV02_MHCI.mgf	Mgf

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Project description:Chlorella has been reported to have various physiological activities, including antiarteriosclerotic, cholesterol-lowering, anti-inflammatory, and immunoregulatory effects. However, there has been no report on the long-term effects of chlorella ingestion on immunity. In the present study, 4- to 10-week-old (young) and 4- to 50-week-old (old) female BALB/c mice were sensitized or not with ovalbumin (OVA), and given basic diet containing chlorella powder at 2% or basic diet alone. The effects of chlorella ingestion on immunity were investigated by measurement of splenic cytokines and immunoglobulin (Ig), analysis of T- and B-cells in the spleen and small intestine by flow cytometry, and analysis of the liver by DNA microarray. Results were compared between the young and old, OVA-sensitized and -nonsensitized, and chlorella and non-chlorella ingestion groups. Production of interferon-γ (IFN-γ) was maintained in the nonsensitized old groups, and ratios of T-helper type 1 (Th1) to T-helper type 2 (Th2) cells were similar in the young and old groups. In addition, overproduction of OVA-specific Igs due to OVA sensitization was strongly suppressed, and significant immunotolerance was exhibited irrespective of age. In addition, suppression of T-cell decreases in the spleen due to aging and suppression of changes in T- and B-cells due to OVA sensitization in the small intestinal lymph were demonstrated on flow cytometric analyses. On DNA microarray analysis, immune-related terms including IL11 and major histocompatibility complex (MHC) class 1 were detected, and expression of genes was shown, which were related to IL1-linked genes and complex involving macrophages from the pathways of cytokines and inflammatory response. In addition, suppressions of declined lipid metabolism and energy production were also suggested. Although how the ingredients in chlorella were involved in these changes is unclear, our findings suggest that prevention of decrease in acquired immunity by aging and induction of strong immunotolerance occurred following chlorella ingestion. The Young Control group and Old Control group were fed normal food, and the Old Chlorella intake group was fed food including 2.0% Chlorella powder. The total RNA was isolated from liver and samples was pooled for each group (n = 6 for each group). A table of normalized log2 ratios (where numerator is sample expression data, and denominator is all sample median data) is linked below as a supplementary file.

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Determining the changes in the immunopeptidome in vivo following treatment with Papaverine

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