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AMP-kinase mediates adaptation of glioblastoma cells to conditions of the tumour microenvironment

ABSTRACT: Proteomic analysis of LNT-299 cells.In this experimental study, we investigated the role of the AMPK-mediated stress response in human GB cells. Mimicking the conditions of the tumour microenvironment we deprived GB cells of glucose and oxygen to characterize metabolic effects and to delineate the relevance of potential downstream targets. AMPK inhibition was modelled by using double-knockout cells of the catalytic subunits α1 and α2. We here report that AMPK activation promotes GB cell survival in the context of both hypoxia and glucose starvation by promoting metabolic adaptation. Furthermore, we found that knockout of the catalytic AMPK subunits resulted in an impaired tumour formation and prolonged survival in vivo. Taken together, the results of this study indicate that the use of AMPK inhibitors could be beneficial to improve GB therapy strategies.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christian Münch

LAB HEAD: Christian Münch

PROVIDER: PXD055976 | Pride | 2024-12-02

REPOSITORIES: Pride

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			Action	DRS
	20230216_LU_LC2_MAA_JBW_LNT229_400ng_F.msf	Msf
	20230216_LU_LC2_MAA_JBW_LNT229_400ng_F.mzML	Mzml
	20230216_LU_LC2_MAA_JBW_LNT229_400ng_F.mzid.gz	Mzid
	20230216_LU_LC2_MAA_JBW_LNT229_400ng_F01.raw	Raw
	20230216_LU_LC2_MAA_JBW_LNT229_400ng_F02.raw	Raw

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