Proteomics

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PDCD6 modulates LC3-associated phagocytosis-mediated antimicrobial defense by regulating lactate metabolism


ABSTRACT: LC3-associated phagocytosis (LAP) is critical in host defense against invading pathogens, but the molecular mechanism for LAP activation is still unclear. Here, we find programmed cell death 6 (PDCD6) as a negative regulator of LAP. PDCD6 deficiency in mice and macrophages induces enhanced bactericidal activity and LAP formation. In parallel, lactate dehydrogenase A (LDHA) activity and lactate production is induced in macrophages challenged with bacteria, Zymosan or Pam3CSK4, while genetic ablation or pharmacological inhibition of LDHA reduces lactate levels and impairs bactericidal activity in vivo and in vitro. Mechanistically, PDCD6 interacts with LDHA to downregulate lactate metabolism, leading to reduced RUBCN lactylation at lysine33 (K33). By contrast, PDCD6-deficiency increases RUBCN lactylation, thereby promotes RUBCN interaction with VPS34, LAP formation, and protective responses. Our results thus suggest a PDCD6-LDHA-lactate-RUBCN axis of innate immunity regulation that may both contribute to protection from infectious diseases and serve as targets for therapeutic development.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Sun Lulu  

LAB HEAD: Tianliang Li

PROVIDER: PXD056047 | Pride | 2024-10-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
GE015GI_EV.msf Msf
GE015GI_EV.raw Raw
GE015GI_PDCD6.msf Msf
GE015GI_PDCD6.raw Raw
checksum.txt Txt
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