Project description:To assess the significant downstream pathways affected by the overexpression of LONELY GUY in Chlorella, transcriptome analysis was carried out using RNA sequencing of three biological replicates each from WT and LOG OX1 at 14 dpi (1h before dark phase). The 14 dpi was chosen for the analysis because it was in the middle of the growth phase, where the slower growth of LOG OX1 was evident.
Project description:Multi-omics analysis of the symbiotic green algae, Chlorella variabilis, revealing the genetic basis of the obligate endosymbiotic lifestyle
Project description:Purpose: The purpose of this study are to identify the miRNA involved in regulating the production of metabolites in Chlorella sorokiniana and Chlorella zofingiensis under normal and stress-induced condition through RNA-sequencing technique. Methods: miRNA transcriptome profile from normal and stress sample of C. sorokiniana and C. zofingiensis were generated, in triplicate, using Illumina Miseq. The sequence reads that passed quality filters were analysed using CLC genomic workbench and OmiRas. Results: The known and predicted novel miRNAs were identified. Although most of the identified miRNAs were not functionally determined, this study suggests that they were species-specific, which may have roles in regulating genes during stress related condition.