ABSTRACT: Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease
Project description:Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease Raw sequence reads
Project description:Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease Raw sequence reads
Project description:MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that regulate targeted mRNAs by degrading or repressing translation, considered as post-transcrption regulators. So far, a large number of miRNAs have been discovered in model plants, but little information is available on miRNAs in banana. In this study, by sequencing the small RNA (sRNA) transcriptomes of Fusarium wilt resistant and susceptible banana varieties, 139 members in 38 miRNA families were discovered, and six out of eight new miRNAs were confirmed by RT-PCR. According to the analysis of sRNA transcriptome data and qRT-PCR verification, some miRNAs were differentially expressed between Fusarium wilt resistant and susceptible banana varieties. Two hundred and ninety-nine and 31 target genes were predicted based on the draft maps of banana B genome and Fusarium oxysporum (FOC1, FOC4) genomes respectively. Specifically, two important pathogenic genes in Fusarium oxysporum genomes, feruloyl esterase gene and proline iminopeptidase gene, were targeted by banana miRNAs. These novel findings may provide a new strategy for the prevention and control of Fusarium wilt in banana.
2016-10-14 | GSE74005 | GEO
Project description:Disease-suppressive and conducive soils of banana Fusarium wilt
| PRJNA1043397 | ENA
Project description:Disease-suppressive and conducive soils of banana Fusarium wilt fungi
Project description:Fusarium oxysporum causes Fusarium wilt syndrome in more than 120 different plant hosts, including globally important crops such as tomato, cotton, banana, melon, etc. F. oxysporum shows high host specificity in over 150 formae speciales and have been ranked in the top 10 plant fungal pathogens. Although three PMTs encoded by the pmt1, pmt2, and pmt4 are annotated in the genome of F. oxysporum, their functions have not been reported. As O-mannosylation is not found in plants, a comprehensive understanding of PMTs in F. oxysporum becomes attractive for the development of new strategy against Fusarium wilt. In order to understand the molecular mechanism of the differential functions of three PMTs, a comparative O-glycoproteome analysis of the pmt mutants were carried out.
Project description:Banana xylem sap contained defense-related proteins, among which HIRP1, E3, CHI, GRP, CXE and GLIP involved in banana defense against TR4. To our knowledge, this is first report to analyze changes in banana xylem sap proteins response to TR4, which help us to explore molecular mechanisms of banana resistant to Fusarium wilt.
