Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease
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PROVIDER: PRJNA432834 | ENA |
REPOSITORIES: ENA
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Project description:Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease Raw sequence reads
| PRJNA432446 | ENA
Project description:MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that regulate targeted mRNAs by degrading or repressing translation, considered as post-transcrption regulators. So far, a large number of miRNAs have been discovered in model plants, but little information is available on miRNAs in banana. In this study, by sequencing the small RNA (sRNA) transcriptomes of Fusarium wilt resistant and susceptible banana varieties, 139 members in 38 miRNA families were discovered, and six out of eight new miRNAs were confirmed by RT-PCR. According to the analysis of sRNA transcriptome data and qRT-PCR verification, some miRNAs were differentially expressed between Fusarium wilt resistant and susceptible banana varieties. Two hundred and ninety-nine and 31 target genes were predicted based on the draft maps of banana B genome and Fusarium oxysporum (FOC1, FOC4) genomes respectively. Specifically, two important pathogenic genes in Fusarium oxysporum genomes, feruloyl esterase gene and proline iminopeptidase gene, were targeted by banana miRNAs. These novel findings may provide a new strategy for the prevention and control of Fusarium wilt in banana.
2016-10-14 | GSE74005 | GEO
Project description:Banana xylem sap contained defense-related proteins, among which HIRP1, E3, CHI, GRP, CXE and GLIP involved in banana defense against TR4. To our knowledge, this is first report to analyze changes in banana xylem sap proteins response to TR4, which help us to explore molecular mechanisms of banana resistant to Fusarium wilt.
2020-03-30 | PXD018261 |
Project description:Nitrogen (N) fertilisers are routinely applied to bananas (Musa spp.) to increase production, but may exacerbate important disease such as Fusarium wilt of banana (FWB). Here, we characterised the effects of N rate and form (ammonium or nitrate) on FWB severity, the banana root proteome, and the diversity of rhizosphere bacterial and fungal communities. Banana plants (Musa ABB) were grown under greenhouse conditions in soil with ammonium or nitrate supplemented at five N rates, and with or without inoculation with Fusarium oxysporum f. sp. cubense (Foc). The growth of non-inoculated plants was positively correlated with N rate. In bananas inoculated with Foc, disease severity increased with N rate, resulting in Foc-inoculated plant growth being greatest at intermediate N rates. The abundance of Foc was weakly related to the treatment conditions and was a poor predictor of disease severity. Fungal diversity was consistently affected by Foc inoculation, while bacterial diversity was associated with changes in soil pH resulting from N addition, in particular ammonium. N rate altered the expression of host metabolic pathways associated with carbon fixation, energy usage, amino acid metabolism, and importantly stress response signalling, irrespective of inoculation or N form. Furthermore, in diseased plants, Pathogenesis-related protein 1, a key endpoint for biotic stress response and the salicylic acid defence response to biotrophic pathogens, was negatively correlated with the rate of ammonium fertiliser but not nitrate. As expected, inoculation with Foc altered the expression of a wide range of processes in the banana plant including those of defence and growth. In summary, our results indicate that the severity of FWB was negatively associated with host defences, which were influenced by N application (particularly ammonium), and shifts in microbial communities in response to ammonium-induced acidification.
2022-04-18 | MSV000089270 | MassIVE
Project description:Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter, Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen (Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43-81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants.
| S-EPMC10437119 | biostudies-literature
Project description:Purpose: Molecular analysis of chickpea-Foc interaction; Methods: Four LongSAGE libraries of wilt-resistant and wilt-susceptible chickpea cultivars prepared after Foc inoculation and sequenced using Ion Torrent PGM. Results: Transcriptome analyses revealed expression of several plant defense and pathogen virulence genes with their peculier expression patterns in wilt-resistant and wilt-susceptible chickpea cultivars. Conclusion: The study identified several candidate Foc resistant genes, which can be used for crop improvement after their functional validation.
2017-08-21 | GSE86377 | GEO
Project description:Discovery and Differential Expression of miRNAs in Fusarium Wilt Resistant and Susceptible Banana Varieties
| PRJNA298728 | ENA
Project description:Bacterial Wilt Disease Suppressive Soil Microbiome Targeted loci cultured
| PRJNA566273 | ENA
Project description:The soil-borne fungal pathogen Fusarium oxysporum f.sp. is responsible for Fusarium wilt. cubense tropical race 4, is one of the most devastating diseases in bananas, regarded as a major yield-reducing factor in the banana industry worldwide. Understanding the molecular interactions in banana defense responses is an important tool to reveal the unexplained processes that underlie banana resistance to Fusarium oxysporum f. sp. cubense tropical race 4. The seedlings of moderately resistant variety Guijiao No. 9 and a susceptible cultivar Guijiao No. 6 were cultured in tissue culture, and the characterize protein profile expression changes responses to after inoculation the Fusarium oxysporum f. sp. of cubense tropical race 4 were detected by isobaric labeling based on MS2 quantification at the 2nd, 4th, 6th and 8th day. Interestingly, new genes in the resistance of banana to Foc37-GFP were identified, including several other serine/threonine-protein kinase, AvrRpt-cleavage domain-containing protein, peptidylprolyl isomerase and some Jacalin-type lectin domain, the resistance-related pathways “ribosome”, “microbial metabolism in diverse environments”,“carbon metabolism”,“biosynthesis of amino acids”and “biosynthesis of antibiotics” pathways were significantly enriched, the resistant banana cultivar Guijiao 9 shows formation of different constitutive cell barriers to restrict spreading of Fusarium oxysporum f. sp. cubense tropical race 4. In this study, the dynamic change root proteomic of moderately resistant cultivar Guijiao 9 and a susceptible cultivar Guijiao 6 were characterized and provided a differentially expressed proteins comparative analysis of the compatible and incompatible interaction between Fusarium oxysporum f. sp. cubense tropical race 4 and banana. These findings provide a substantial contribution to existing sequence resources for banana, and a strong basis for future proteomic research. The proteins that displayed two-fold changes in intensity are related to biochemical processes that may be differentially altered at various times after Fusarium oxysporum f. sp. cubense tropical race 4 infection. These findings will accelerate research on resistance in banana to Fusarium oxysporum f. sp. cubense tropical race 4 and contribute to a better understanding of the banana defense mechanism to plant pathogens, hopefully.
2024-08-29 | PXD055439 |