Project description:The goal of the project is to determine proteomic changes occuring in Arabidopsis thalina plants overexpressing Phospholipid:diacylglycerol acyltransferase 1 (PDAT1) gene. This modification resulted in prolongation of plant lifespan together with profound increase of cold and hot tolerance, asdescribed in Demski et.al, 2020. In this study we have compared Arabidopsis leaf proteomes of wild-type plandt and two independently created overexpressor strains grown in normal conditions.
Project description:The scope of the project is to find DNA binding sites of the DNA binding protein AtDEK3. For the experiments AtDEK3 overexpressor (OE) plants have been obtained. The AtDEK3 was tagged with a CFP-tag. With ChIP the protein was pulled down and eluted DNA was send for sequencing. Gene ontology (GO) term classification of genes enriched for AtDEK3 binding indicated that AtDEK3 target genes are involved in diverse biological processes.