ABSTRACT: Ruminococcus gnavus was isolated from human faecal samples of a healthy adult human individual. This study aims to investigate mucin degradation by focussing on the utilisation of terminal mucin glycans.
Project description:We performed a transcriptome analysis of Ruminococcus gnavus with and without 0.1% (w/w) agarooligosaccharide exposure to validate the mechanism of growth inhibition.
Project description:Strains of Ruminococcus gnavus, specifically ATCC 29149, ATCC 35913, H2-28, and Mediterraneibacter gnavus AGR 2154 (previously known as Ruminococcus gnavus), were cultured under three different conditions:
Meat Broth (CNTRL)
Meat Broth with Sialic Acid (SIALIC)
Meat Broth with 1% Porcine Mucin (PORCINE)
The cultures were incubated for 3 hours. Following incubation, the spent media of the strains underwent polar metabolite analysis using liquid chromatography-mass spectrometry (LC-MS) in negative ion mode.
Project description:Strains of Ruminococcus gnavus, specifically ATCC 29149 and ATCC 35913, as well as Mediterraneibacter gnavus H2-28 and AGR 2154 (formerly known as Ruminococcus gnavus), were cultured under three different conditions:
Meat Broth (CTRL)
Meat Broth with Sialic Acid (SIALIC)
Meat Broth with 1% Porcine Mucin (PORCINE)
The cultures were incubated for 3 hours. Following incubation, the spent media from each strain underwent polar metabolite analysis using liquid chromatography-mass spectrometry (LC-MS) in positive ion mode.
Project description:Symbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health and disease. Some gut bacteria utilize mucus as a nutrient source and can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how Ruminococcus torques—a known mucin-degrader that remains poorly studied despite its implication in inflammatory bowel diseases (IBDs)— degrades mucin glycoproteins or their component O-linked glycans to understand its effects on the availability of mucin-derived nutrients for other bacteria. We found that R. torques utilizes both mucin glycoproteins and released oligosaccharides from gastric and colonic mucins, degrading these substrates with a panoply of mostly constitutively expressed, secreted enzymes. Investigation of mucin oligosaccharide degradation by R. torques revealed strong fucosidase, sialidase and b1,4-galactosidase activities. There was a lack of detectable sulfatase and weak β1,3-galactosidase degradation, resulting in accumulation of glycans containing these structures on mucin polypeptides. While the Gram-negative symbiont, Bacteroides thetaiotaomicron grows poorly on mucin glycoproteins, we demonstrate a clear ability of R. torques to liberate products from mucins, making them accessible to B. thetaiotaomicron. This work underscores the diversity of mucin-degrading mechanisms in different bacterial species and the probability that some species are contingent on others for the ability to more fully access mucin-derived nutrients. The ability of R. torques to directly degrade a variety of mucin and mucin glycan structures and unlock released glycans for other species suggests that it is a keystone mucin degrader, which may contribute to its association with IBD.
Project description:Strain Ruminococcus gnavus TSDC16.1-1.1 (species Ruminococcus gnavus) was isolated from the fecal microbiota of a USA female at time point zero . The species name was assigned by genome clustering.
Project description:Strain Ruminococcus gnavus TSDC16.1-2.1 (species Ruminococcus gnavus) was isolated from the fecal microbiota of a USA female at time point zero . The species name was assigned by genome clustering.
Project description:Strain Ruminococcus gnavus TSDC16.1-3.1 (species Ruminococcus gnavus) was isolated from the fecal microbiota of a USA female at time point zero . The species name was assigned by genome clustering.
Project description:This study investigates the growth of Ruminococcus gnavus strain ATCC 29149 under two different conditions over a period of 3 hours. The experimental conditions include:
RG (Ruminococcus gnavus in Meat Broth): R. gnavus grown in standard meat broth.
RS-SA (Ruminococcus gnavus in Meat Broth with Sialic Acid): R. gnavus grown in meat broth supplemented with sialic acid.
Control conditions were also established for comparison:
CT (Control in Meat Broth): Standard meat broth without R. gnavus.
CT-SA (Control in Meat Broth with Sialic Acid): Meat broth with sialic acid, also without R. gnavus.
