ABSTRACT: Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Sprint-2 nitrogen-fixing nodules (3 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Frisson nitrogen-fixing nodules (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Finale nitrogen-fixing nodules (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Sparkle nodulated roots (2 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed NGB1238 nodulated roots (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:This study aims to compare the potential of standard RNA-sequencing (RNA-Seq) and 3’ massive analysis of c-DNA ends (MACE) RNA-sequencing for the analysis of fresh tissue and describes transcriptome profiling of formalin-fixed paraffin-embedded (FFPE) archival human samples by MACE.
Project description:Plants of the resistant Pisum sativum subsp. syriacum accession P665 and the susceptible pea cultivar Messire were inoculated with M. pinodes.The experiment was conducted in three replicates. 16, 24 and 48 hours after inoculation RNA was isolated from leaves of infected plants and transcribed into cDNA. For each time point and replicate, Cy-labelled cDNA samples from resistant and susceptible plants were mixed and hybridized to Mt16kOLI1Plus microarray
Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.
Project description:We studied potentially amyloidogenic proteins (e.g. protein forming polymers and complexes that are resistant to treatment with ionic detergents) in root nodules formed by two lines of garden pea (P. sativum L.): Sprint-2 (Fix+ phenotype) and Sprint-2Fix- (sym31) (Fix- phenotype) inoculated with the Rhizobium leguminosarum bv. viciae RCAM1026 root nodule bacteria. The Fix+ phenotype is characterized by effective (ability to fix nitrogen) root nodules formation. The Fix- line is a descendant of the Fix+ line and forms ineffective root nodules (unable to fix nitrogen) with undifferentiated bacteroids. We demonstrated the presence of both plant and bacterial proteins in detergent resistant fractions, including previously identified amyloid proteins RopA and RopB of R. leguminosarum and vicilin of P. sativum L.
Project description:We sequenced the transcriptomes using massive analysis of cDNA ends (MACE) of whole blood samples from 25 healthy volunteers and 47 PDAC patients to identify mRNAs potentially allowing for discrimination between both groups
Project description:We investigated the transcriptome dynamics of Brassica oleracea in response to Xcc race 1 infection at 3 and 12 days after inoculation by using Massive Analysis of 3′-cDNA Ends (MACE) technology