Shell DNA
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB20113 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1894870.fastq.gz | Fastqsanger.gz | |||
| ERR1894880.fastq.gz | Fastqsanger.gz | |||
| ERR1894890.fastq.gz | Fastqsanger.gz | |||
| ERR1894900.fastq.gz | Fastqsanger.gz | |||
| ERR1894910.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 73 |
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Project description:Advancing responsible genomic Analyses of ancient mollusc shells
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Project description:In this project we have applied palaeoproteomics to study archaeological shell beads dating back to the beginning of the 7th mill. cal BCE and recovered from a prehistoric site in Jordan (Ba'ja). These beads were made of mollusc shells and were of unknown origin, but suspected to be made of larger bivalves such as Tridacna or Spondylus sp. The aim of the study was to analyse ancient proteins extracted from these beads in order to infer their biological origin. Tiny amounts of ancient samples were used and the extracted proteins were analysed by high resolution liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). In order to identify obtained peptides, we have created an ‘in-house’ molluscan protein database, by gathering molluscan sequences (proteins and transcriptomes), which were downloaded from publicly available databases in NCBI. Searching against this comprehensive database, we were able to identify a number of shell proteins from Tridacna sp., the giant clam shells. Our study reports the oldest molluscan protein sequences ever recovered that come from a very warm environment.
2024-11-04 | PXD041082 | Pride
Project description:Unveiling the Ecological Applications of Ancient DNA from Mollusk Shells
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Project description:Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process of matrix-assisted biomineralization. Despite recent advances, many parts of the biomineralization process and its evolutionary origin remain a mystery. The pearl oyster Pinctada fucata martensii is a well-known master of biomineralization, but the molecular mechanisms underlie its production of remarkable shells and pearls is not fully understood. We sequenced the highly polymorphic genome of the pearl oyster and conducted multi-omic and biochemical studies to probe nacre formation. We identified a large set of novel proteins participating in matrix-framework formation, many in expanded families, including components similar to that found in vertebrate bones such as collagen-related VWA-containing proteins (VWAP), chondroitin sulfotransferases and regulatory elements.Considering that there are only collagen-based matrices in vertebrate bones and chitin-based matrices in most invertebrate skeletons, the presence of both chitin and elements of collagen-based matrices in nacre matrices suggests that elements of chitin- and collagen-based matrices are deeply rooted and might be part of an ancient biomineralizing matrix. Our results expand the current shell matrix-framework model and provide new insights into the evolution of diverse biomineralization systems.
2017-07-07 | PXD006786 | Pride
Project description:Palaeoproteomics analysis of mollusc shells (Modiolus modiolus, Ostrea edulis, Unio pictorum, Margaritifera margaritifera, Unio crassus, Pseudunio auricularius) and shell ornaments (double-buttons) from Neolithic European sites (Havnø, Hornstaad-Hörnle IA, Peştera Ungurească).
2019-05-08 | PXD011985 | Pride
Project description:This study investigates the degradation of mineral-associated proteins from Spondylus gaederopus mollusc shell. We analysed intracrystalline proteins extracted from powdered shell samples that were artificially aged by heating at 110 °C for different time durations (0, 1, 2, 4, 8, 16, 24, 48, 96, 240 h). TMT-labelled quantitative proteomics was used to study the degradation pathways and to identify peptide bonds that were unstable and prone to hydrolysis.
2021-11-25 | PXD025068 | Pride
Project description:The appearance of hard mineralized exoskeletons is a critical leap for animal evolution and partially lead to the explosion of diverse animals during the Cambrian, for example, molluscs. A majority of molluscs have mineralized shells to protect themselves. Despite numerous studies that have studied the remarkable mechanical properties of shells, the origin of shell formation is still elusive. Hence, this study investigated the overlooked shell proteome of chitons, which belong to polyplacophoran, Aculifera of Mollusca. By comparing the shell proteome to well-studied Conchifera groups, we inferred possible ancestral biomineralization toolkits of stem-group Mollusca. Taking advantage of the recently sequenced chiton mantle transcriptome and genome, eight core biomineralization proteins were identified by proteomics. Surprisingly, in contrast to previous thought that shell formation is convergently evolved, two important shell matrix proteins, Nacrein-like and Pif-like proteins were found to be conserved among Aculifera and Conchifera groups. Our findings identify a missed link of mineralized shell evolution in Mollusca and pose a hypothesis that stem-group molluscs have already evolved core biomineralization toolkits, which likely facilitate the formation of mineralized shells for protection that partially leads to their explosion.
2023-01-03 | PXD033490 | Pride
Project description:Molluscan shells are diverse, beautiful, and mechanically robust. They are commonly used during ancient human life as money, decorative objects, or functional tools. To reconstruct their origin is important for us to understand their production process as well as underlying cultural contexts. Luodian, a unique shell art in China since 3000 years ago, has served as a cultural carrier between China and Europe. This study combined proteomics and microstructural analysis to identify the raw materials origin of three Luodian samples from Qing Dynasty with species resolution and potential age levels. Shell proteomics and multiple sequence alignment have identified key shell matrix proteins (N66 and Pif) to be of Pinctada maxima, a pearl oyster distributed in the indo-pacific regions. After establishing that the thickness of nacreous tablets in the shell is inversely proportional to the shell size for the first time, we proposed to infer the age of raw materials in different Luodian samples, which are tailored to different purposes. This study provides a framework and foundation for identifying the origin of various shell arts especially made from pearl oysters.
2022-11-22 | PXD038290 |
Project description:Molluscan larval ontogeny is a highly conserved process typical of 3 principal developmental stages. A characteristic unique to each of these stages is shell design, termed prodissoconch I, prodissoconch II and dissoconch. These shells vary in morphology, mineralogy and microstructure. The discrete temporal transitions in shell biomineralization between these larval stages are utilized in this study to investigate transcriptional involvement in several distinct biomineralization events. Scanning electron microscopy and X-ray diffraction analysis of P. maxima larvae and juveniles collected throughout post-embryonic ontogenesis, document the mineralogy and microstructure of each shelled stage as well as establishing a timeline for transitions in biomineralization. P. maxima larval samples most representative of these biomineralization distinctions and transitions were analyzed for differential gene expression on the microarray platform PmaxArray 1.0. A number of transcripts are reported as differentially expressed in correlation to the mineralization events of P. maxima larval ontogeny. Some of those isolated are known shell matrix genes while others are novel, these are discussed in relation to potential shell formation roles. This interdisciplinary investigation has married the shell developments of P. maxima larval ontogeny with corresponding gene expression profiles, furthering the elucidation of shell biomineralization. Keywords: Temporal expression profiling by array
2010-02-05 | GSE14305 | GEO
Project description:Molluscan larval ontogeny is a highly conserved process typical of 3 principal developmental stages. A characteristic unique to each of these stages is shell design, termed prodissoconch I, prodissoconch II and dissoconch. These shells vary in morphology, mineralogy and microstructure. The discrete temporal transitions in shell biomineralization between these larval stages are utilized in this study to investigate transcriptional involvement in several distinct biomineralization events. Scanning electron microscopy and X-ray diffraction analysis of P. maxima larvae and juveniles collected throughout post-embryonic ontogenesis, document the mineralogy and microstructure of each shelled stage as well as establishing a timeline for transitions in biomineralization. P. maxima larval samples most representative of these biomineralization distinctions and transitions were analyzed for differential gene expression on the microarray platform PmaxArray 1.0. A number of transcripts are reported as differentially expressed in correlation to the mineralization events of P. maxima larval ontogeny. Some of those isolated are known shell matrix genes while others are novel, these are discussed in relation to potential shell formation roles. This interdisciplinary investigation has married the shell developments of P. maxima larval ontogeny with corresponding gene expression profiles, furthering the elucidation of shell biomineralization. Keywords: Temporal expression profiling by array Microarray is used to examine the temporal differential expression of transcripts from several bivalve larval development stages including 24hrs post fertilization, 3 days, 17 days, 20 days, 23 days, 26 days, 30 days, 35 days, 40 days. Differential expression profiles for transcripts of all the temporal samples was determined based on comparison to a common reference of unfertilized eggs. Each temporal larval sample included in the study has at least 3 replicate hybridizations. Dye flips have been incorporated in the replicates. A total of 46 microarray hybridizations were performed in this investigation for differential expression analysis.
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