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Harvest of the oleaginous microalgae Scenedesmus obtusiusculus from optimized salt water culture by flocculation

ABSTRACT: Harvest of the oleaginous microalgae Scenedesmus obtusiusculus from optimized salt water culture by flocculation

PROVIDER: PRJEB22943 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2164511_1.fastq.gz	Fastqsanger.gz
	ERR2164511_2.fastq.gz	Fastqsanger.gz
	ERR2164512_1.fastq.gz	Fastqsanger.gz
	ERR2164512_2.fastq.gz	Fastqsanger.gz
	ERR2164513_1.fastq.gz	Fastqsanger.gz

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Project description:Transcriptome analysis of Oleaginous microalgae from the Peruvian Amazon of the genus Ankistrodesmus, Chlorella, and Scenedesmus Transcriptome

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Harvest of the Oleaginous Microalgae Scenedesmus obtusiusculus by Flocculation From Culture Based on Natural Water Sources.

Project description:Harvest and dewatering poses a significant economical burden for industrial algae biomass production. To mitigate these effects, energy efficient techniques for these process steps have to be developed. Flocculation of the microalgae Scenedesmus obtusiusculus in salt based medium was induced by pH-shift and alternatively by addition of two biological flocculants, chitosan, and the commercial tannin CFL-PT. This is the first time that CFL-PT is used as an algae flocculant particularly focusing on harvesting of halophilic strains. The method was characterized and subsequently optimized. In comparison to biological flocculants, induction by pH shift is far cheaper, but due to buffering effects of the brackish cultivation medium infeasible amounts of base are required to raise the pH-value. tannin appears to be superior compared to chitosan not only in the absence of algae organic matter (AOM), but tannin-based harvest is also more robust regarding culture pH in presence of AOM. A higher flocculant-demand for modified tannin compared to chitosan is offset by the lower price. Given the employed strain and cultivation conditions, cultivation time had no pronounced effect on flocculation efficiencies (FE) while algae zeta-potential and bacterial communities also remained stable.

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The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus

Project description:Daphnia infochemicals can induce defensive responses in microalgae Scenedesmus spp., including colony formation and flocculation. We investigated the phenomenon using a combination of microalgal biology, physiological ecology, and quantitative isobaric tagging (iTRAQ) proteomics. We have discovered that flocculation as a defense response occurs at the early ‘alarm’ phase and requires increased energy resources, with an important role proposed in cysteine synthesis. It appears to be initially stimulated by the production of an extracellular matrix (mainly composed ofby polysaccharides and fatty acids), and later sustained at the ‘acclimation’ stage through Mitogen-activated protein kinase (MAPK) signalling cascades. Defensive strategies leading to colony formation required investment into fatty acids metabolism, to ensure the accurate separation of membranes during cell division. Furthermore, higher energy demands were required at the alarm phase which subsequently decreased at the acclimation stage, thus suggesting a trade-off between colony formation and support of floc form.

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2013-09-01 | E-GEOD-43385 | biostudies-arrayexpress

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