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Next-generation sequence analysis reveals methicillin-resistance transfer to a methicillin susceptible Staphylococcus aureus (MSSA) strain that subsequently caused a methicillin resistant Staphylococcus aureus (MRSA) outbreak: a descriptive study.

ABSTRACT: Next-generation sequence analysis reveals methicillin-resistance transfer to a methicillin susceptible Staphylococcus aureus (MSSA) strain that subsequently caused a methicillin resistant Staphylococcus aureus (MRSA) outbreak: a descriptive study.

PROVIDER: PRJEB24708 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2275540_1.fastq.gz	Fastqsanger.gz
	ERR2275540_2.fastq.gz	Fastqsanger.gz
	ERR2275535_1.fastq.gz	Fastqsanger.gz
	ERR2275535_2.fastq.gz	Fastqsanger.gz
	ERR2275536_1.fastq.gz	Fastqsanger.gz

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Proteomic differences in methicillin-sensitive and methicillin-resistant Staphylococcus aureus

Project description:To establish general differences between the protein expression in S. aureus strains, five methicillin sensitive S. aureus (MSSA) strains and five methicillin resistant S. aureus (MRSA) strains were compared both individually and as MSSA and MRSA groups in the absence of antibiotics. Proteins were compared by ultra-performance liquid chromatography-mass spectrometry.

2023-04-25 | PXD021629 | Pride

Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus

Project description:Purpose: Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non-healthcare environments. Experiment design: Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected. Results: 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up-regulated and 156 down-regulated. Differentially abundant proteins were predominantly involved in catalytic and binding activity. Among 164 proteins that had differences in abundance, 29 proteins were involved in pathogenesis, antimicrobial activities，stress response, mismatch repair and cell wall synthesis. Twenty-two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up-regulated in MRSA. Moreover, the up-regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host. Conclusions: Enhanced pathogenicity, antimicrobial activity and stress response were observed in MRSA compared to MSSA.

2020-01-09 | PXD014478 | Pride

proteomic and metabolomic profiling of methicillin resistant and methicillin sensitive Staphylococcus aureus using a simultaneous extraction protocol

Project description:understanding the biology of methicillin resistant staphylococcus aureus (MRSA) is crucialto unlocking insights for new targets in the fight against this pathogen, there is howeverlimited reports of methadological approaches for carrying proteomic and metabolomic profiling in S.aureus. Therefore, we describe the use of a dual-functionality methanol extraction method for the concurrent extraction of protein and metabolites from S.aureus and reporton the comparative analysis of the proteomic and metabolomic profiles of MRSA versus methicillin sensitive S. aureus (MSSA). Using a reference strain from MRSA and MSSA, we first compared the MRSA proteome extracted using the methanol method to the one from the traditionally used urea method. Then using the methanol extraction method, we compared the proteome and metabolome of MRSA versus MSSA. Through this study, we demonstrated the effectivnessof the methanol-based-dual-extraction method, providing simultaneous insights into the proteomic and metabolomic landscapes of S.aureus strains, demonstrating the utility of proteomic and metabolomic profiling for elucidating the biological basis of antimicrobial resistance

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Mapping disease-associated regulatory circuits by cell type from single-cell multiomics data (S. aureus scRNA-seq)

Project description:Resolving chromatin remodeling-linked gene expression changes is important for understanding disease states. We describe MAGICAL (Multiome Accessible Gene Integration Calling And Looping), a hierarchical Bayesian approach that leverages paired scRNA-seq and scATAC-seq data from different conditions to map disease-associated transcription factors, regulatory sites and genes as regulatory circuits. By introducing hidden Bayesian variables to allow modeling noise and signal variation across cells and conditions in both transcriptome and chromatin data, in systemic evaluations MAGICAL achieved high accuracy on circuit prediction at cell-type resolution. We applied MAGICAL to study Staphylococcus aureus sepsis from peripheral blood mononuclear single cell data that we generated from infected subjects and healthy uninfected controls. MAGICAL identified sepsis-associated regulatory circuits predominantly in CD14 monocytes, known to be sepsis activated. We addressed the challenging problem of distinguishing methicillin-resistant- (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) infections, where differential expression analysis failed to show predictive value. MAGICAL, however, identified epigenetic circuit biomarkers that distinguished MRSA from MSSA.

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Mapping disease-associated regulatory circuits by cell type from single-cell multiomics data (S. aureus scATAC-seq)

2023-05-19 | GSE220188 | GEO

Mapping disease-associated regulatory circuits by cell type from single-cell multiomics data (COVID-19 scATAC-seq)

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Potential Mechanism of Action of 3'-Demethoxy-6-O-Demethylisoguaiacin on Methicillin-resistant Staphylococcus aureus

2015-06-27 | GSE70309 | GEO

Potential Mechanism of Action of 3'-Demethoxy-6-O-Demethylisoguaiacin on Methicillin-resistant Staphylococcus aureus

Project description:Methicillin-resistant Staphylococcus aureus is one of the major causative agents associated to infections with a high morbidity and mortality in hospitals worldwide. In previous studies, we reported that lignan 3'-demethoxy-6-O-demethylisoguaiacin isolated and characterized from Larrea tridentata showed the best activity towards methicillin-resistant S. aureus. Understanding of mechanism of action of drugs allows design drugs in a better way. Therefore, we employed microarray to obtain gene expression profile of methicillin-resistant S. aureus after exposure to 3'-demethoxy-6-O-demethylisoguaiacin. The results showed that lignan had an effect on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This study consisted of comparison of isolated RNA of MRSA not treated and MRSA treated with lignan 3'-demethoxy-6-O-demethylisoguaiacin. Both RNAs samples were differentially dyed with Cy3 and Cy5 during cDNA synthesis and hybridized on DNA chip. Afterwards, the chip was scanned in a GenePix 4000B scanner. The resulting gene expression profile was analyzed in databases for functional annotations to find a potential mechanism of the lignan in MRSA.

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CodY - The impact of CodY on virulence determinant production in community-associated methicillin-resistant Staphylococcus aureus

Project description:The Impact of CodY on Virulence Determinant Production in Community-Associated Methicillin Resistant Staphylococcus aureus

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Proteomic differences between methicillin resistant Staphylococcus aureus showing synergistic effects with cannabinoids

Project description:Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen in both community and health care settings, which causes a wide range of infections. Its resistance to β-lactam antibiotics and methicillin in particular, greatly complicates treatment options and success rate due to the limited number of antibiotics with activity against MRSA. To further the development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. Cannabinoid compounds including cannabidiol (CBD), tetrahydrocannabinol (THC) and cannabinol (CBN) have shown promise as potential antibiotic adjuvants. In the present study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds, and subsequently analysed using metaproteomics to assess the metabolic response. Subjecting MRSA to methicillin made the cells more viable and increased their energy production, as well as upregulation of penicillin-binding protein 2 (PBP2). The cannabinoids all showed antimicrobial activity against MRSA, and inhibited the energy production of the cells as well as PBP2 when used in combination with methicillin. Furthermore, all three cannabinoid compounds inhibited resistance mechanisms in MRSA, resulting in a decrease in the minimum inhibitory concentration (MIC) of methicillin when used in combination.

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