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Embryonic mouse mammary progenitor cells +/-Sox9

ABSTRACT: Embryonic mouse mammary progenitor cells +/-Sox9

PROVIDER: PRJEB27130 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2612020_1.fastq.gz	Fastqsanger.gz
	ERR2612020_2.fastq.gz	Fastqsanger.gz
	ERR2612021_1.fastq.gz	Fastqsanger.gz
	ERR2612021_2.fastq.gz	Fastqsanger.gz
	ERR2612022_1.fastq.gz	Fastqsanger.gz

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Embryonic mouse mammary progenitor cells +/-Sox9

Project description:CRISPR/Cas9-mediated gene targeting of Sox9 from embryonic mammary progenitors cell line eMPC1. Part of a study to assess the effect of Sox9 ablation on embryonic mammary progenitor cell fate and function.

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Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells.

Project description:Embryonic mammary cells are a unique population comprised of undifferentiated, highly plastic progenitor cells that create normal mammary tissues. The mammary gland continues to develop after birth from descendants of embryonic mammary cells. Here, we establish cell lines from mouse mammary organs, immediately after they formed during prenatal development, to facilitate studies of primitive mammary cells, which are difficult to isolate in sufficient quantities for use in functional experiments. We show that some lines can be induced to secrete milk, a distinguishing feature of mammary epithelial cells. Targeted deletion of Sox9, from one clone, decreases the ability to respond to lactogenic stimuli, consistent with a previously identified role for Sox9 in regulating luminal progenitor function. Sox9 ablation also leads to alterations in 3D morphology and downregulation of Zeb1, a key epithelial-mesenchymal transition regulator. Prenatal mammary cell lines are an invaluable resource to study regulation of mammary progenitor cell biology and development.

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Project description:RNA-seq of embryonic mammary progenitor cell lines. Embryonic mammary progenitor cell (eMPC) clones were derived from E12.5-stage mammary organs. After microdissection, mammary primordial 3 was cultured on thick basement membrane extract for 4 weeks. After enzymatic dissociation eMPC swere plated and expanded in 2D culture to obtain a pool of eMPCs. eMPC cells were subject to single cell sorting using FACS. Clones were expanded as single-cell derived clones (eMPC1, 2, etc.) to create the eighteen single cell-derived clones described in this study.

2018-11-30 | E-MTAB-6856 | biostudies-arrayexpress

E-MTAB-6846

Project description:Embryonic mouse mammary progenitor cells +/-Sox9

2018-09-17 | E-MTAB-6846 | ExpressionAtlas

Bulk RNA-seq of mouse embryonic progenitor cells

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Profiling of Sox9-related genes in chondrogenesis using Sox9-3'EGFP knock-in and Sox9-EGFP/EGFP null chimeric mouse embryos

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Microarray evaluation of in vitro cultured Sox9+ mouse lung progenitors isolated from E12.5 embryonic lung progenitors

Project description:The study aims to show that Sox9+ Chd1+ mouse embryonic lung progenitors can be isolated and expanded long-term in 3D culture while maintaining their multipotency. in vitro cultured Sox9+ Chd1+ lung progenitors transcriptionally resemble their in vivo counterparts and show significant difference from adult lung epithelial (Cdh1+ and EpCAM+) and non-epithelial (Cdh1- and EpCAM-) cells

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