Embryonic mouse mammary progenitor cells
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ABSTRACT:
PROVIDER: PRJEB22485 | ENA |
REPOSITORIES: ENA
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Project description:Embryonic mouse mammary progenitor cells +/-Sox9
| PRJEB27130 | ENA
Project description:CRISPR/Cas9-mediated gene targeting of Sox9 from embryonic mammary progenitors cell line eMPC1. Part of a study to assess the effect of Sox9 ablation on embryonic mammary progenitor cell fate and function.
2018-11-30 | E-MTAB-6846 | biostudies-arrayexpress
Project description:The mammary epithelium depends on specific lineages and their stem and progenitor function to accommodate hormone-triggered physiological demands in the adult female. Perturbations of these lineages underpin breast cancer risk, yet our understanding of normal mammary cell composition is incomplete. Here, we build a multimodal resource for the adult gland through comprehensive profiling of primary cell epigenomes, transcriptomes, and proteomes. We define systems-level relationships between chromatin–DNA–RNA–protein states, identify lineage-specific DNA methylation of transcription factor binding sites, and pinpoint proteins underlying progesterone responsiveness. Comparative proteomics of estrogen and progesterone receptor–positive and –negative cell populations, extensive target validation, and drug testing lead to discovery of stem and progenitor cell vulnerabilities. Top epigenetic drugs exert cytostatic effects; prevent adult mammary cell expansion, clonogenicity, and mammopoiesis; and deplete stem cell frequency. Select drugs also abrogate human breast progenitor cell activity in normal and high-risk patient samples. This integrative computational and functional study provides fundamental insight into mammary lineage and stem cell biology.
PMID: 29921600 (Table S5 and Table S7)
| MSV000079330 | MassIVE
Project description:RNA-seq of embryonic mammary progenitor cell lines. Embryonic mammary progenitor cell (eMPC) clones were derived from E12.5-stage mammary organs. After microdissection, mammary primordial 3 was cultured on thick basement membrane extract for 4 weeks. After enzymatic dissociation eMPC swere plated and expanded in 2D culture to obtain a pool of eMPCs. eMPC cells were subject to single cell sorting using FACS. Clones were expanded as single-cell derived clones (eMPC1, 2, etc.) to create the eighteen single cell-derived clones described in this study.
2018-11-30 | E-MTAB-6856 | biostudies-arrayexpress
Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis
2018-08-02 | MSV000082680 | GNPS
Project description:Low input proteomics on FABP7 hi/low mammary luminal progenitor cells isolated from human patient samples
2023-12-11 | MSV000093639 | MassIVE
Project description:RNA-seq of embryonic mammary progenitor cell lines in compari (part A)
| PRJEB27161 | ENA
Project description:The mammary primordium represents the earliest evidence of commitment to the mammary lineage. The primordium forms via inductive tissue interactions between its constitutive tissues, the mesenchyme and epithelium. Here, we describe an analysis of the transcriptome of the mammary bud epithelium and its associated mesenchyme, two distinct cellular compartments that comprise the mammary primordium. Using network analysis, we found candidate mediators of mammary cell fate, differentiation and progenitor cell function that signal from mammary lineage inception during embryogenesis through postnatal development. Genetic features of mammary primordial cells overlapping with human breast progenitor cells identified potential regulators of key progenitor cell functions conserved across species. These results provide new insights into genetic regulatory mechanisms of mammary and in particular novel regulators of stromal-epithelial communications.
2011-11-11 | E-TABM-1099 | biostudies-arrayexpress
Project description:Mammary alveologenesis is abrogated in the absence of the transcription factors STAT5A/5B that mediate cytokine signaling. To reveal the underlying causes for this developmental block we studied mammary stem and progenitor cells. While loss of STAT5A/5B did not affect the stem cell population and their ability to form mammary ducts, luminal progenitors were greatly reduced and unable to form alveoli during pregnancy. Temporally-controlled expression of transgenic STAT5A in mammary epithelium lacking STAT5A/5B restored the luminal progenitor population and rescued alveologenesis in a reversible fashion in vivo. Taken together, STAT5A is necessary and sufficient for the establishment of luminal progenitor cells. The mammary tissues from two mice of each genotype were collected 6 days (sample 9 and 11 were WT)
2009-10-21 | E-GEOD-17723 | biostudies-arrayexpress
Project description:Bovine mammary stem cells (MaSC) are a source of ductal and lobulo-alveolar tissue during development of mammary gland and its remodeling in repeating lactation cycles. We hypothesize that the number of MaSC, their molecular properties and interactions with their niche may be essential to determine the mammogenic potential in heifers. To verify this hypothesis we compared the number of MaSC and transcriptomic profile in mammary tissue of 2-year-old, non-pregnant dairy (Holstein-Friesian) and beef (Limousin) heifers. For identification and quantification of putative stem/progenitor cells in mammary tissue sections scanning cytometry was used with a new combination of MaSC molecular markers: stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B) protein. Double labeled cells were located mainly in the basal layers of mammary epithelium. Cytometric analysis of Sca-1pos FNDC3Bpos cells revealed significantly higher number in HF (2.94M-BM-10.35%) than in LM (1.72M-BM-10.20%) heifers. More advanced development of mammary tissue in HF heifers was accompanied by higher expression of intramammary hormones, growth factors, cytokines, chemokines and transcription regulators. The model of transcriptomic niche favorable for MaSC was associated with regulation of genes involved in MaSC maintanence, self renewal, proliferation, migration, differentiation, mammary tissue remodeling, angiogenesis, regulation of adipocyte differentiation, lipid metabolism and steroid and insulin signaling. In conclusion the high mammogenic potential in postpubertal dairy heifers is facilitated by a higher number of MaSC and up-regulation of mammary auto-, paracrine factors representing MaSC niche. Keywords: stem/progenitor cells, transcriptomics, mammary gland, dairy and beef heifers Two-condition experiment, LIM vs. HF. Pulled quarters of mammary glands form 10 LIM heifers (test) and 10 HF heifers (reference). Sample 3 and 4 are dye swaps.
2013-06-16 | E-GEOD-47816 | biostudies-arrayexpress