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LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes - sequencing data

ABSTRACT: LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes - sequencing data

PROVIDER: PRJEB2754 | ENA |

REPOSITORIES: ENA

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LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes

Project description:Drosophila melanogaster KC cells were treated with RNAi against dL(3)mbt, dLint1 or EGFP (control) and the resulting changes in gene expression were measured.

2012-11-01 | E-MTAB-849 | biostudies-arrayexpress

LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes.

Project description:Mutations in the l(3)mbt tumour suppressor result in overproliferation of Drosophila larval brains. Recently, the derepression of different gene classes in l(3)mbt mutants was shown to be causal for transformation. However, the molecular mechanisms of dL(3)mbt-mediated gene repression are not understood. Here, we identify LINT, the major dL(3)mbt complex of Drosophila. LINT has three core subunits-dL(3)mbt, dCoREST, and dLint-1-and is expressed in cell lines, embryos, and larval brain. Using genome-wide ChIP-Seq analysis, we show that dLint-1 binds close to the TSS of tumour-relevant target genes. Depletion of the LINT core subunits results in derepression of these genes. By contrast, histone deacetylase, histone methylase, and histone demethylase activities are not required to maintain repression. Our results support a direct role of LINT in the repression of brain tumour-relevant target genes by restricting promoter access.

| S-EPMC3342951 | biostudies-literature

LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes - sequencing data

Project description:dLint1 (CG1908) was ChIPseq`d in Drosophila melanogaster KC cells and S2 cells

2012-06-27 | E-MTAB-855 | biostudies-arrayexpress

ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

Project description:We employed CRISPR/Cas-mediated genome editing to generate S2 cell lines expressing GFP-tagged dCoREST, dL(3)mbt, dLSD1 and dG9a, respectively. This allowed us to determine the genome-wide binding profiles for these proteins by ChIP-seq using the same antibody (anti-GFP) in each case.

2019-11-04 | E-MTAB-8341 | biostudies-arrayexpress

Blastocystis sp. DL isolate:DL

Project description:Blastocystis sp. DL isolate:DL Genome sequencing

| PRJNA1084795 | ENA

Pseudomonas sp. DL strain:DL

Project description:Pseudomonas sp. DL strain:DL Genome sequencing

| PRJNA1186688 | ENA

The Drosophila cancer-germline, head-to-head gene pair TrxT and dhd is dispensable for normal brain development but plays a major role in l(3)malignant brain tumour growth.

Project description:Drosophila thioredoxins Deadhead (Dhd) and Thioredoxin-T (TrxT) are encoded by a head-to-head gene pair in the X chromosome. Akin to human cancer-germline (CG) genes, dhd and TrxT expression is normally sex and germline specific, but becomes upregulated in brain tumours of either sex caused by mutation in l(3)malignant brain tumour (l(3)mbt). Using CRISPR/Cas9-mediated knock-out alleles and RNA-seq, we show that while both TrxT and dhd are dispensable for normal brain development, each of them has a significant but partial effect on l(3)mbt brain tumour development as well as on tumour-linked transcriptomic signatures. Moreover, this effect is enhanced by the concomitant removal of both thioredoxins that strongly inhibits l(3)mbt larval brain tumour traits, reduces gene expression differences between tumour and wild-type tissue, and essentially erases all traces of gene expression differences between tumours from larvae of different sex. These results show that TrxT and dhd play a major synergistic role in the emergence of l(3)mbt tumour-linked transcriptomic signatures and tumour development, which is remarkable taking into account that these two genes are never expressed together under normal conditions. We have also found that TrxT is crucial but dhd has no effect on the growth of male-derived l(3)mbt allografts, hence suggesting that the initial stages of tumour development and sustained, long-term tumour growth may depend upon different molecular pathways. In humans, head-to-head inverted gene pairs are abundant among the CG genes that map to the X chromosome, some of which have been shown to work as a unit cancer. Our results identify the first instance of an X-linked, head-to-head CG gene pair in Drosophila and underpin the potential of such CG genes that are dispensable for normal development and homeostasis of somatic tissue, as targets to curtail malignant growth with minimal impact on overall health.

2024-07-17 | GSE263157 | GEO

ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

Project description:ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

| PRJEB34474 | ENA

shotgun LC/MS/MS of L(3)mbt-IP samples using OSCs

Project description:L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed shotgun LC/MS/MS of L(3)mbt-IP samples and identified a new interactor in OSCs.

2022-08-15 | PXD026945 | Pride

Transcription profiling of mouse fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5

Project description:Gene expression in the fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5 were analyzed using Affymetrix Genechip to assess the effect of the gene knock-out on hematopoietic cells.

2004-11-30 | E-MEXP-219 | biostudies-arrayexpress

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