Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

ABSTRACT: ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

PROVIDER: PRJEB34474 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3528410.fastq.gz	Fastqsanger.gz
	ERR3528420.fastq.gz	Fastqsanger.gz
	ERR3528430.fastq.gz	Fastqsanger.gz
	ERR3528411.fastq.gz	Fastqsanger.gz
	ERR3528421.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

Project description:We employed CRISPR/Cas-mediated genome editing to generate S2 cell lines expressing GFP-tagged dCoREST, dL(3)mbt, dLSD1 and dG9a, respectively. This allowed us to determine the genome-wide binding profiles for these proteins by ChIP-seq using the same antibody (anti-GFP) in each case.

2019-11-04 | E-MTAB-8341 | biostudies-arrayexpress

RNA-seq of D. melanogaster S2 cells upon depletion of dCoREST and its interacting partners compared to control

Project description:RNAi KD of dCoREST, dLSD1, dL(3)mbt, dLint-1, dG9a and EGFP (control) followed by determination of gene transcription changes by RNA-seq

2019-01-01 | E-MTAB-7440 | biostudies-arrayexpress

LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes

Project description:Drosophila melanogaster KC cells were treated with RNAi against dL(3)mbt, dLint1 or EGFP (control) and the resulting changes in gene expression were measured.

2012-11-01 | E-MTAB-849 | biostudies-arrayexpress

Genome-wide study of the role of the histone demethylase dLsd1 in oogenesis

Project description:To study the effect of dLsd1 knockdown on gene expression in oogenesis, we performed transcripts profiling by microarray in wild type ovaries and ovaries depleted for dLsd1.

2019-11-21 | GSE110751 | GEO

Genome-wide study of the role of the histone demethylase dLsd1 in oogenesis

Project description:The histone demethylase LSD1 is a key chromatin regulator that plays a crucial role in development. It has been shown to act both as a co-repressor and a co-activator of genes expression and to affect the expression of many genes important for development. In Drosophila, dLsd1 is essential for oogenesis; however, our current knowledge of dLsd1 function is insufficient to explain its precise role in the ovary. To better define the role of dLsd1 in oogenesis, we performed genome-wide analysis of dLsd1 binding in the ovary by Chromatin Immunoprecipitation followed by sequencing (ChIP-Seq).

2019-02-08 | GSE110331 | GEO

Transposable elements silencing by the histone demethylase dLSD1 is required to achieve normal wing size

Project description:Determination of tissue size depends on the proper response of cells to a wide array of external and internal signals. Chromatin acts as a platform/substrate of signal integration and storage. Signal transduction pathways converge upon chromatin modifying enzymes and transcription factors to reprogram gene expression. However, little is known about the role of chromatin modifying factors in determining tissue size. Here, we show that loss of function of the histone demethylase dLSD1 results in a significant reduction of Drosophila wing size due to a decreased cell number. We show that dLSD1 depletion affects proliferation and induces DNA damage in larval wing imaginal discs. Through transcriptomics analysis, we find that dLSD1 controls the expression of multiple gene networks in wing imaginal discs. We then show that normal dLsd1 function contributes to repressing Transposable Element (TE) transcription and mobility. Additionally, our results suggest that increased TE mobility due to dLsd1 depletion contributes directly to altered organ size.

2025-02-11 | GSE244445 | GEO

Pseudomonas sp. DL strain:DL

Project description:Pseudomonas sp. DL strain:DL Genome sequencing

| PRJNA1186688 | ENA

Blastocystis sp. DL isolate:DL

Project description:Blastocystis sp. DL isolate:DL Genome sequencing

| PRJNA1084795 | ENA

LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes - sequencing data

Project description:LINT - a novel dL(3)mbt containing complex represses malignant brain tumour signature genes - sequencing data

| PRJEB2754 | ENA

Drosophila melanogaster Set8 and L(3)mbt function in gene expression independently of histone H4 lysine 20 methylation [GFP-L3mbt_CUT&RUN]

Project description:Mono-methylation of Lysine 20 of histone H4 (H4K20me1) is catalyzed by Set8 and thought to play important roles in many aspects of genome function that are mediated by H4K20me-binding proteins. We interrogated this model in a developing animal by comparing in parallel the transcriptomes of Set8null, H4K20R/A, and l(3)mbt mutant Drosophila melanogaster. We found that the gene expression profiles of H4K20A and H4K20R larvae are markedly different than Set8null larvae despite similar reductions in H4K20me1. Set8null mutant cells have a severely disrupted transcriptome and fail to proliferate in vivo, but these phenotypes are not recapitulated by mutation of H4K20 indicating that the developmental defects of Set8null animals are largely due to H4K20me1-independent effects on gene expression. Further, the H4K20me1 binding protein L(3)mbt is recruited to the transcription start sites of most genes independently of H4K20me even though genes bound by L(3)mbt have high levels of H4K20me1. Moreover, both Set8 and L(3)mbt bind to purified H4K20R nucleosomes in vitro. We conclude that gene expression changes in Set8null and H4K20 mutants cannot be explained by loss of H4K20me1 or L(3)mbt binding to chromatin, and therefore that H4K20me1 does not play a large role in gene expression.

2024-06-07 | GSE268820 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data