ABSTRACT: The endemic Cladophorales (Ulvophyceae) of ancient Lake Baikal represent a monophyletic group of very closely related but morphologically diverse species
Project description:Color is an important trait in nature, playing a role in selection and speciation. The most important colorants in crustaceans are carotenoids, which in complexes with carotenoid-binding proteins provide an astonishing variety of colors from red to violet. Over 350 species and subspecies of amphipods (Crustacea: Amphipoda) endemic to Lake Baikal exhibit an impressive variability of colors and coloration patterns. However, the mechanisms forming this diversity are underexplored. In this work, we analyze the coloration of two species of endemic Lake Baikal amphipods, Eulimnogammarus cyaneus and E. vittatus. These species are brightly colored and, even more importantly, characterized by intraspecific color variability. We showed that the color of either species strongly correlated with the abundance of two putative carotenoid-binding proteins (the relative abundance of these proteins was higher in blue or teal-colored animals than in the orange- or yellow-colored ones.). With LC-MS/MS, we were able to identifiy these proteins, which turned out to be similar to the pheromone/odorant-binding protein family.
Project description:Hemocytes are blood cell circulating in the hemolymph and playing important role in crustacean immunity. On the one side this cells functions as phagocytes and express immune compounds in the external milieu on the other side. To find out the protein composition of hemocytes, we obtained hemocytes proteome of Baikal endemic amphipod Eulimnogammarus verrucosus for the first time. For this, a proteomic analysis of the protein extract were performed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). A total of 1152 unique proteins were discovered. Hemocyanin/phenoloxidase, actin and tubulin were detected in proteome in the greatest amount. Also we discovered proteins of the C-type lectin superfamily, which are probably involved in the molecular pattern recognition during immune response.
Project description:In this project we aimed to establish a proteome-based pipeline for a non-model Baikal endemic amphipod species Eulimnogammarus cyaneus (Dybowsky, 1874). We used next-generation transcriptome sequencing to create a database for protein identification and investigated the proteome of E. cyaneus with LC-MS with particular emphasis on sexual dimorphism and response to acute thermal stress. We were able to characterize ca. 1000 protein groups and found male- and female-specific proteins, as well as proteins specific for thermal stress conditions.
Project description:In this work, we describe the transcriptional profiles of adapted and non-adapted one-month-old Baikal whitefish juveniles after heat shock exposure. Preadapted fish were exposed to a repeated thermal rise of 6 °C above control temperature every 3 days throughout embryonic development. One month after hatching, preadapted and non-adapted larvae were either maintained at control temperatures (12 °C) or exposed to an acute thermal stress (TS) of 12 °C above control temperature. The information on transcriptional profiles will contribute to further understanding of the mechanisms of adaptation of whitefish to the environment.
Project description:Transcriptome sequencing of two Lake Baikal endemic (Eulimnogammarus verrucosus and E. cyaneus) and one Holarctic (Gammarus lacustris) transcriptome species from the Baikal region subjected to different stressors
| PRJNA529908 | ENA
Project description:Endemic lake Baikal amphipods Raw sequence reads
