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Abscisic acid (ABA) application to Populus × canescens in hydroponics

ABSTRACT: Abscisic acid (ABA) application to Populus × canescens in hydroponics

PROVIDER: PRJEB30670 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3047700.fastq.gz	Fastqsanger.gz
	ERR3047710.fastq.gz	Fastqsanger.gz
	ERR3047720.fastq.gz	Fastqsanger.gz
	ERR3047701.fastq.gz	Fastqsanger.gz
	ERR3047711.fastq.gz	Fastqsanger.gz

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Abscisic acid (ABA) application to Populus × canescens in hydroponics

Project description:4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatment (+ABA) and discontinuous 100 µM ABA treatment (±ABA). ABA was fed to +ABA plants during the whole treatment period of 30 days. ABA was fed to ±ABA plants for three days in two weeks. Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. RNA was extracted from these samples and followed by RNA-sequencing.

2019-01-20 | E-MTAB-7587 | biostudies-arrayexpress

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Project description:Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added cycloheximide to preferentially obtain immediate targets of ABA addition. We believe that using a sensitized background of an ABA auxotroph would yield a set of genes that are very closely regulated by ABA at the transcriptional level. This data set will be used for network analysis of ABA signaling.

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Project description:The softened green berries of Cabernet Sauvignon were cultured in a Petri dish. 0.3M sucrose solution with (±)-abscisic acid (ABA) and without (±)-ABA (control) were applied to the Petri dishes. The berry skins were sampled at 3 and 10 days after the application. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV17 at PLEXdb. ABA treated - time: 3 days(2-replications); ABA treated - time: 10 days(2-replications); Non-treated (control) - time: 3 days(2-replications); Non-treated (control) - time: 10 days(2-replications)

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Project description:Heterotrimeric G proteins mediate crucial and diverse signaling pathways in eukaryotes. To gain insights into the regulatory modes of the G protein and the co-regulatory modes of the G protein and the stress hormone abscisic acid (ABA), we generated and analyzed gene expression in G protein subunit single and double mutants of the model plant Arabidopsis thaliana. Through a Boolean modeling approach, our analysis reveals novel modes of heterotrimeric G protein action. Keywords: transcriptome analysis; G protein subunit mutants; abscisic acid (ABA) Microarray data were generated from four genotypes (wild type, gpa1-4 mutant, agb1-2 mutant, agb1-2 gpa1-4 double mutant) with or without ABA treatment. Arabidopsis plants were grown in growth chambers with an 8 hr light/16hr dark. Three hundred Arabidopsis leaves excised from 60-70 five-week-old plants were used as the starting material for each guard cell microarray. Ten mature leaves taken from 3-4 plants grown side-by side with the plants for guard cell isolation were used for each leaf sample. Excised leaf and isolated guard cell samples were treated with ABA (50 μM) or EtOH (solvent control) for 3 hrs. For each type of sample (guard cells or leaves), three independent biological replicates were performed, resulting in a total of 48 microarray hybridizations (2 sample types ´ 4 genotypes ´ two treatments ´3 replicates).

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