Project description:The transcriptional changes produced in B. abortus by growth in the presence of erythritol have been analyzed by RNAseq. Erythrtol is a sugar believed to have a relation with the capability of Brucella to produce abortions in ruminants.
Project description:RNA from B. abortus 2308 grown in BB or BB+ erythritol, was labelled and hybridized with the ORFeome microarray to determine transcriptional changes produced by erythritol
Project description:The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source.
Project description:MavR is a 160 nucleotide regulatory RNA molecule that is produced during B. abortus strain 2308 growth in nutrient-replete broth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic mavR mutant cells.
Project description:The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source. Two control samples (glucose) and two experimental samples (erythritol) were analyzed on Nimblegen B. melitensis microarray chips.
Project description:Long-term consumption of erythritol, a widely used sugar substitute, has been associated with increased risks of thrombosis and cardiometabolic diseases. In this study, we investigated the effects and mechanisms of allulose in mitigating these risks compared to erythritol using the clusterProfiler tool. Since a high-fat diet (HFD) is known to enhance platelet aggregation, we compared the pathways related to these processes between groups of mice treated with allulose and those treated with erythritol. While erythritol exacerbated HFD-induced increased platelet aggregation, allulose treatment significantly reduced it. The groups consisted of a normal diet group (ND, with 10% of calories derived from fat), a high-fat diet group (HFD, with 40% of calories from fat), a high-fat diet with 5% allulose supplementation (ALLU), and a high-fat diet with 5% erythritol supplementation (ERY).
Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)
Project description:Isogenic deletion and truncation of specific genes encoding RNases in Brucella abortus were analyzed for changes in gene expression. The main goal of this work is to determine the mRNAs that exhibit dysregulation when small regulatory RNAs (i.e., Bsr8) or RNases (i.e., RNaseE and RNaseJ) are invactivated in Brucella abortus. Small regulatory RNAs often control gene expression by binding directly to mRNAs to block translation or induce their degradation, and RNA from a deletion of one sRNA gene, bsr8, was analyzed to uncover the mRNAs that may be controlled by BsrB. RNases are enzymes that cleave RNAs during processing, turnover, and regulatory events, and RNaseE and RNaseJ appear to be important for B. abortus virulence. Therefore, to determine the mRNAs potentially targetd by these RNases, RNA from a strain harboring a RNaseE truncation and a strain carrying a deletion of rnaseJ were analyzed. In the end, the objective of this study was to gain insight into the regulatory patterns of specific B. abortus sRNAs and RNases.
Project description:abcR1 and abcR2 produce two regulatory RNA molecules, that are produced during stationary phase growth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic abcR1 abcR2 double mutant cells.
Project description:We performed a transcriptome analysis of Staphylococcus pseudintermedius with and without 5% (w/w) erythritol exposure to validate the mechanism of growth inhibition.
