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RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

ABSTRACT: RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

PROVIDER: PRJEB31729 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3224474_1.fastq.gz	Fastqsanger.gz
	ERR3224474_2.fastq.gz	Fastqsanger.gz
	ERR3224475_1.fastq.gz	Fastqsanger.gz
	ERR3224475_2.fastq.gz	Fastqsanger.gz
	ERR3224476_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

Project description:More than 5 g/L ethanol was detected at 84 h in the late-exponential phase with 0.1 MPa at pH 6.0 in the batch fermentation of C. ljungdahlii grown autotrophically. Interestingly, ethanol started to be reassimilated in the stationary phase. In order to understand the metabolic flux of ethanol, comparative transcriptome between exponential and stationary phases were performed.

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Energy conservation and carbon flux arrangement in the fermentation of Clostridium ljungdahlii with CO or CO2/H2

Project description:Clostridium ljungdahlii not only utilizes CO, but also H2 as energy source during autotrophic growth. In theory, CO is a more energetically and thermodynamically favourable energy source than H2 in the gas fermentation of C. ljungdahlii. However, how C. ljungdahlii conserves energy for growth and ethanol/acetate formation grown on CO or CO2/H2 is not in great detail. In this study, C. ljungdahlii was fermented on CO and CO2/ H2 at pH 6.0 with 0.1 MPa gas pressure. C. ljungdahlii produced 27 g/L acetate, 9 g/L ethanol, 8 g/L 2,3-butanediol and traces of lactate in the presence of CO as energy source, while it produced 25.8  0.1 g/L acetate, 1.8  0.1 g/L ethanol, 0.7  0.01g/L 2,3-butanediol and trances of lactate in the same fermentation condition using H2 as energy source. Therefore, comparative transcriptomes between cells grown on CO and cells grown on H2/CO2 were performed to investigate gene expression profiles based on three biological replicates.

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