Ontology highlight
ABSTRACT:
PROVIDER: PRJEB32939 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR3363850_1.fastq.gz | Fastqsanger.gz | |||
| ERR3363850_2.fastq.gz | Fastqsanger.gz | |||
| ERR3363860_1.fastq.gz | Fastqsanger.gz | |||
| ERR3363860_2.fastq.gz | Fastqsanger.gz | |||
| ERR3363870_1.fastq.gz | Fastqsanger.gz |
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Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.
2021-05-14 | GSE114386 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE11437: Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array GSE11514: Expression QTL mapping of Toxoplasma gondii genes, Tachyzoite array Keywords: SuperSeries Refer to individual Series
2008-05-22 | E-GEOD-11515 | biostudies-arrayexpress
Project description:To date little is known about the transcriptome of Hammondia hammondi, the nearest extant relative of Toxoplasma gondii. In this study we used an existing microarray to query Toxoplasma gondii and Hammondia hammondi transcript abundance in sporulated oocysts.
2014-10-02 | GSE61963 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE25468: Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii. GSE25469: Expression data from WT or p65-/- mouse embryonic fibroblasts (MEFs) infected with Toxoplasma gondii. Refer to individual Series
2011-01-10 | E-GEOD-25476 | biostudies-arrayexpress
Project description:The in vitro effect of infection with different strains of Toxoplasma gondii was tested 24 hours after infection of Human Foreskin Fibroblasts (HFF) The strains tested include RH, VEG, and transgenic strains of RH overexpressing ROP38 or ROP21 Total RNA of Toxoplasma gondii infected HFF cell was compared to uninfected cells
2010-07-31 | E-GEOD-22402 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE26558: Expression Quantitative Trait Locus (eQTL) Mapping of Stage-specific Gene Expression in Progeny from a type I X III Genetic Cross of Toxoplasma gondii GSE26607: Genomic hybridizations for the parents and progeny of the Toxoplasma gondii I X III genetic cross Refer to individual Series
2012-01-11 | E-GEOD-26608 | biostudies-arrayexpress
Project description:Expression profile microarray of human foreskin fibroblast cell comparing control untreated HFF cell with HFF cell infected with ME49 strain.Study on Toxoplasma gondii infection of HFF cell LncRNAs expression, for further studies on the differential exprssion of LncRNAs in HFF cell against the infection of Toxoplasma gondii research provide the basic function.
2017-01-01 | GSE92603 | GEO
Project description:To identify accessible chromatin regions in the human host cells during Toxoplasma parasite infection (uninfected, RH-infected and Pru-infected human foreskin fibroblasts) and in the obligate intracellular parasite Toxoplasma gondii (Type 1 RH strain and Type 2 Pru strain), ATAC-seq was performed.
2022-09-30 | GSE209866 | GEO
Project description:Tymoshenko2015 - Genome scale metabolic model
- ToxoNet1
This model is described in the article:
Metabolic Needs and
Capabilities of Toxoplasma gondii through Combined
Computational and Experimental Analysis.
Tymoshenko S, Oppenheim RD, Agren R,
Nielsen J, Soldati-Favre D, Hatzimanikatis V.
PLoS Comput. Biol. 2015 May; 11(5):
e1004261
Abstract:
Toxoplasma gondii is a human pathogen prevalent worldwide
that poses a challenging and unmet need for novel treatment of
toxoplasmosis. Using a semi-automated reconstruction algorithm,
we reconstructed a genome-scale metabolic model, ToxoNet1. The
reconstruction process and flux-balance analysis of the model
offer a systematic overview of the metabolic capabilities of
this parasite. Using ToxoNet1 we have identified significant
gaps in the current knowledge of Toxoplasma metabolic pathways
and have clarified its minimal nutritional requirements for
replication. By probing the model via metabolic tasks, we have
further defined sets of alternative precursors necessary for
parasite growth. Within a human host cell environment, ToxoNet1
predicts a minimal set of 53 enzyme-coding genes and 76
reactions to be essential for parasite replication.
Double-gene-essentiality analysis identified 20 pairs of genes
for which simultaneous deletion is deleterious. To validate
several predictions of ToxoNet1 we have performed experimental
analyses of cytosolic acetyl-CoA biosynthesis. ATP-citrate
lyase and acetyl-CoA synthase were localised and their
corresponding genes disrupted, establishing that each of these
enzymes is dispensable for the growth of T. gondii, however
together they make a synthetic lethal pair.
This model is hosted on
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and identified by:
MODEL1504280000.
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2015-07-07 | MODEL1504280000 | BioModels