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Ehux_minicosm

ABSTRACT: Natural Bacterial Communities Associated with Emiliania huxleyi

PROVIDER: PRJEB33262 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3408980_1.fastq.gz	Fastqsanger.gz
	ERR3408980_2.fastq.gz	Fastqsanger.gz
	ERR3408981_1.fastq.gz	Fastqsanger.gz
	ERR3408981_2.fastq.gz	Fastqsanger.gz
	ERR3408972_1.fastq.gz	Fastqsanger.gz

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Transcriptional response of Emiliania huxleyi CCMP2090 exposed to the bacterial signaling molecule 2-heptyl-4-quinolone

Project description:In order to determine how the bacterial quorum-sensing precursor molecule 2-heptyl-4-quinolone (HHQ) induces cellular senescence in populations of some eukaryotic marine phytoplankton, we examined the transcriptional response of Emiliania huxleyi CCMP2090 to HHQ exposure. Quadruplicate batch cultures were treated with HHQ at three concentrations (1, 10 and 100 ng/ml) and compared to a vehicle control after 24 and 72 hours of exposure. The majority of gene expression changes occurred after 24 hr of exposure in the 10 and 100 ng/ml treatments and many of these changes persisted for 72 hr in the 100 ng/ml treatment. RNAseq analysis revealed that HHQ exposure impacts many aspects of Emiliania huxleyi metabolism including: chlorophyll biosynthesis and light harvesting, vitamin biosynthesis, vesicle trafficking, and general signals related to energy production. Changes in genes involved in intraphase cell cycle check points implicate DNA damage as the primary driver of HHQ-induced cellular senescence.

2020-07-30 | GSE131846 | GEO

Ehux life-cycle stages under N-limitation

Project description:Transcription profiling of haploid and diploid Emiliania huxleyi growing into N limitation

2014-10-01 | E-MTAB-2274 | biostudies-arrayexpress

Comparative genomic hybridization of Emiliania huxleyi to investigate the genetic diversity

Project description:Investigation of the genetic diversity of Emiliania huxleyi, genomic DNA from 15 different strains were compared with the genomic DNA of the sequenced E. huxleyi strain CCMP1516. Gephyrocapsa oceanica and Isochrysis galbana as phylogenetic closely related taxa were used as out-groups.

2010-03-01 | E-MEXP-2388 | biostudies-arrayexpress

Unveiling the transcriptional features associated with coccolithovirus infection of natural Emiliania huxleyi blooms.

2011-11-22 | GSE24341 | GEO

Emiliania huxleyi

Project description:Emiliania huxleyi

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Emiliania huxleyi coccosphere associated proteins identification

Project description:The project aim was to identify proteins associated with the coccosphere of Emiliania huxleyi.

2023-04-25 | PXD027440 | Pride

Emiliania huxleyi BOF92 and Emiliania huxleyi virus99B1

Project description:Emiliania huxleyi BOF92 and Emiliania huxleyi virus99B1 Epigenomics

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Unveiling the transcriptional features associated with coccolithovirus infection of natural Emiliania huxleyi blooms.

Project description:Lytic viruses have been implicated in the massive cellular lysis observed during algal blooms, through which they assume a prominent role in oceanic carbon and nutrient flows. Despite their impact on biogeochemical cycling, the transcriptional dynamics of these important oceanic events is still poorly understood. Here, we employ an oligonucleotide microarray to monitor host (Emiliania huxleyi) and virus (coccolithovirus) transcriptomic features during the course of E. huxleyi blooms induced in seawater-based mesocosm enclosures. Host bloom development and subsequent coccolithovirus infection was associated with a major shift in transcriptional profile. In addition to the expected metabolic requirements typically associated with viral infection (amino acid and nucleotide metabolism, as well as transcription- and replication-associated functions), the results strongly suggest that the manipulation of lipid metabolism plays a fundamental role during host-virus interaction. The results herein reveal the scale, so far massively underestimated, of the transcriptional domination that occurs during coccolithovirus infection in the natural environment. Six mesocosm enclosures were placed in the Raunefjorden (Western Norway coast) and filled with natural community water (in June 2008). Nutrient enrichment was applied in order to trigger the development of E. huxleyi blooms. The major transcriptomic features of those blooms and consequent viral infections were monitered through the use of an oligo microarray containing a total of 3571 gene probes; 2271 (63.6%) matching E. huxleyi ESTs, and 1300 (36.4%) matching EhV-86 and EhV-163 genomic sequences. Each microarray contains 5 technical replicates. Sampling of total RNA present in 2L of water (from each enclosure) was performed once a day from day 8 to day 16. For enclosures 2 and 3 other sampling points were taken, covering the complete dial-cycle (6h,12h,18h, and 24h).

2011-11-22 | E-GEOD-24341 | biostudies-arrayexpress

Transcription profiling of Emiliania huxleyi after 6, 12 and 24 hours of viral infection infected with the virus EhV-86 in comparison to an unifected culture

Project description:Gene expression analysis of Emiliania huxleyi after 6, 12 and 24 hours of viral infection infected with the virus EhV-86 in comparison to an unifected culture.

2009-12-01 | E-MEXP-2382 | biostudies-arrayexpress

Emiliania huxleyi

Project description:Emiliania huxleyi transcriptome

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