Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNAseq of 12-week old AKIMBA versus WT mouse retina

ABSTRACT: Single cell RNAseq of 12-week old AKIMBA versus WT mouse retina

PROVIDER: PRJEB38302 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single cell RNAseq of 12-week old AKIMBA versus WT mouse retina

Project description:Single-cell RNA sequencing was performed on retinal tissue from 12-week-old wild-type and Akimba (Ins2AkitaxVEGF+/-) mice, which are known to replicate features of clinical diabetic retinopathy. The aim of this study was to provide deeper insight into the complex network of molecular and cellular changes that underlie diabetic retinopathy by measuring the transcriptional changes that occur in the different cellular compartments of the degenerating diabetic mouse retina. Retinas (n=4 for Akimba, n=2 for wild-type) were isolated in ice-cold Dulbecco’s Modified Eagle Medium. After rinsing with Dulbecco’s Phosphate-Buffered Saline containing 2% fetal bovine serum, each retina was incubated with 1mL digestion buffer (2mg/mL collagenase-P, 200U/mL DNAse-I (Sigma-Aldrich) in M199 medium (Life Technologies) at 37°C for 10min. Retinal tissue was further dissociated by trituration and the suspension was filtered through a 40µm cell strainer and centrifuged for 5min at 300xg (4°C). Pooled retinal single-cell suspensions from wild-type and Akimba were counted on a Luna-FL Cell Counter (Logos Biosystems) and libraries were prepared with the Chromium Single-cell 3’ V2 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics) aiming for 5000 cells per library. Barcoded libraries were sequenced on an Illumina HiSeq4000 in 25-8-98 paired-end configuration. The transcriptome data for 9474 retinal cells were analysed, yielding 15 clusters corresponding to eight distinct retinal cell types.

2020-05-23 | E-MTAB-9061 | biostudies-arrayexpress

Gene expression profile of grafts in 6-week-old and 12-week-old recipients 24 h after receiving similar donor kidneys

Project description:To investigate the effect of youthful systemic milieu on acute kidney injury in a rat kidney transplantation model based on young (6-week-old) and adult (12-week-old) recipients receiving similar donor kidneys (12-week-old)

2023-06-20 | GSE226680 | GEO

12 Week Rat Jejunum

Project description:12-week-old rats, iron-replete or iron-deficient. Three groups of each condition. 230A and 230B chips used with cRNA derived from jejunal scrapes. Keywords: ordered

2005-07-11 | GSE2269 | GEO

Adrenal gland gene expression from adult (12-week old) hypertensive BPH and adult (12-week old) hypotensive BPL mice

Project description:The objective of the experiment is to determine the genes differentially expressed in the adrenal gland of adult (12-week) BPH and BPL mice.

2010-06-08 | GSE19342 | GEO

RNA sequencing in 12-week old orbital frontal cortex in circHomer1/Homer1b knockdown and control mice

Project description:These are RNA sequencing data from 12 week old orbital frontal cortex from mice who received a shRNA targeting circHomer1 and Homer1b (double knockdown) or control shRNA (scramble)

2022-01-01 | GSE160874 | GEO

Gene expression profile of grafts in 6-week-old and 12-week-old recipients 24 h after receiving similar donor kidneys

Project description:Gene expression profile of grafts in 6-week-old and 12-week-old recipients 24 h after receiving similar donor kidneys

| PRJNA941227 | ENA

Adrenal gland gene expression from adult (12-week old) hypertensive BPH and adult (12-week old) hypotensive BPL mice

Project description:The objective of the experiment is to determine the genes differentially expressed in the adrenal gland of adult (12-week) BPH and BPL mice. Adrenal glands from adult (12-week) hypertensive BPH mice (n=3) and adult (12-week) hypotensive BPL mice (n=3).

2010-06-16 | E-GEOD-19342 | biostudies-arrayexpress

Gene expression in the islets of 10 day old, 4 week old and 12 week old Non-Obese Diabetic (NOD) mice vs. healthy, age-matched NOD.B10 control mice

Project description:Gene expression in the islets of 10 day old, 4 week old and 12 week old Non-Obese Diabetic (NOD) mice vs. healthy, age-matched NOD.B10 control mice

| PRJNA627352 | ENA

Expression data from hippocampus and cortex of 6- and 12-week curcumin-treated 15-month-old rats

Project description:Curcumin has been demonstrated to have many neuroprotective properties, including improvement of cognition in humans and neurogenesis in animals, yet the mechanism of such effects remains unclear. Here, we assessed behavioural performance and hippocampal cell proliferation in aged rats after 6- and 12-week curcumin-fortified diets. Curcumin enhanced non-spatial and spatial memory, as well as dentate gyrate cell proliferation as compared to control diet rats. We also investigated underlying mechanistic pathways that might link curcumin treatment to increased cognition and neurogenesis via microarray analysis of cortical and hippocampal mRNA transcription. We used microarrays to investigate the effects of short-term (6-week) and long-term (12-week) curcumin-supplemented diet on gene expression of hippocampus and cortex in aged rats. The hippocampus and cortex of every three rats from one group were pooled together, respectively and used for RNA extraction and hybridization on Affymetrix microarrays. To ensure the reliability of the data, we conducted hybridization experiments in duplicate microarrays from each RNA sample. The tissues examined by microarray are as follows: the hippocampus and cortex of 6-week curcumin-treated 15-month-old rats, the hippocampus and cortex of 6-week no curcumin-treated 15-month-old rats (control rats), the hippocampus and cortex of 12-week curcumin-treated 15-month-old rats, the hippocampus and cortex of 12-week no curcumin-treated 15-month-old rats (control rats).

2011-10-21 | E-GEOD-33137 | biostudies-arrayexpress

Ligilactobacillus murinus strain:41-week-old WT mice feces | isolate:41-week-old WT mice feces

Project description:Ligilactobacillus murinus strain:41-week-old WT mice feces | isolate:41-week-old WT mice feces Genome sequencing

| PRJNA1036701 | ENA