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RNA-seq of Streptococcus thermophilus N4L grown in two different yeast extracts

ABSTRACT: RNA-seq of Streptococcus thermophilus N4L grown in two different yeast extracts

PROVIDER: PRJEB39803 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4439070.fastq.gz	Fastqsanger.gz
	ERR4439080.fastq.gz	Fastqsanger.gz
	ERR4439090.fastq.gz	Fastqsanger.gz
	ERR4439100.fastq.gz	Fastqsanger.gz
	ERR4439071.fastq.gz	Fastqsanger.gz

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RNA-seq of Streptococcus thermophilus N4L grown in two different yeast extracts

Project description:In the present work, we aimed at investigating how the respective composition (especially the peptide content) of two different yeast extracts influenced Streptococcus metabolism during growth. To reach that goal, we used one strain of S. thermophilus (N4L) and two yeast extract-based growth media leading to similar fermentation kinetics and yields that differed by their peptide content. Three replicate cultures were performed for each yeast extract-based growth medium, under pH control. Samples were collected after 3, 4 and 5 h of growth, cells were harvested

2020-08-10 | E-MTAB-9434 | biostudies-arrayexpress

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

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Project description:We observed the expression profile of the total mRNA of ttcsp1 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Keywords: temperature comparison

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mRNA expression in wild-type of Thermus thermophilus HB8 strain grown on a rich (TT) medium at 45˚C and 70˚C

Project description:We observed the expression profile of the total mRNA of wild-type of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 45˚C compared with that of 70˚C. Keywords: Growth temperature

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Project description:In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase.

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:ENZO)

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Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Experiment Overall Design: wild-type T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in thewild-type cell between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

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