Project description:DNA methylation is a conserved epigenetic mark that is involved in many biological processes including the ripening process of various crop species bearing fleshy fruit, such as tomato, strawberry, and orange. The dynamics and importance of DNA methylation during grapefruit ripening, has however not yet been characterized. In this study, we observed an increase in DNA methylation level during grape berry ripening. The application of a DNA methylation inhibitor delayed ripening, consistent with an important role for DNA methylation in grape berries. In addition, we characterized the methylomes of the flesh and the skin the two main tissues of grape berry pericarp, as they present very distinct metabolic features. Results indicate that grape berry skin has a lower DNA methylation level than the flesh. Whereas in both tissues ripening is associated with an increase in DNA methylation, methylation patterns are clearly distinct between these two tissues as are the metabolic and transcriptomic profiles. Results indicated that the DNA methylation changes are associated with tissue specific metabolic modifications that occur during ripening, and involved in the synthesis of secondary metabolites.
Project description:DNA methylation is a conserved epigenetic mark that is involved in many biological processes including the ripening process of various crop species bearing fleshy fruit, such as tomato, strawberry, and orange. The dynamics and importance of DNA methylation during grapefruit ripening, has however not yet been characterized. In this study, we observed an increase in DNA methylation level during grape berry ripening. The application of a DNA methylation inhibitor delayed ripening, consistent with an important role for DNA methylation in grape berries. In addition, we characterized the methylomes of the flesh and the skin the two main tissues of grape berry pericarp, as they present very distinct metabolic features. Results indicate that grape berry skin has a lower DNA methylation level than the flesh. Whereas in both tissues ripening is associated with an increase in DNA methylation, methylation patterns are clearly distinct between these two tissues as are the metabolic and transcriptomic profiles. Results indicated that the DNA methylation changes are associated with tissue specific metabolic modifications that occur during ripening, and involved in the synthesis of secondary metabolites.
Project description:To gain a comprehensive understanding of non-histone methylation during grape berry ripening. A 4D-label free quantitative proteomics approach was used to study the non-histone methylation of lysine during grape berry development and ripening. The ‘Kyoho’ grape berries at EL 31, EL 33, EL 35 and EL 37 were collected from six trees with equal vigor were selected in spring 2022 at the vineyard of the Henan University of Science and Technology, Luoyang, Henan, China (34.66° N, 112.37° E).
Project description:Taste and color, which are important organoleptic qualities of grape berry, undergo rapid and substantial changes during development and ripening. In this study, we use two cultivars ‘Sanbenti’ and its bud sport ‘11-06-25’ to explore expression profiles differences and identify genes associated with total soluble solid (TSS) and total anthocyanins during grape berry development stages using RNA sequencing.
Project description:Red blotch disease causes major reprogramming of grape berry metabolism and development leading to the inhibition of ripening pathways and stress responses
Project description:The vacuole occupies a large portion of plant cell volume, it is especially true to fruit tissues. Berry flesh cell vacuole serves as storage organelle for water, sugars, acids, secondary metabolites and others, which largely determining berry quality (Fontes et al., 2011a, b; Shiratake and Martinoia, 2007, Conde et al., 2006). However, the molecular basis of these compartmentation processes is still poorly understood. As in many species, the major bottle neck to study these aspects in grapevine is to obtain highly purified vacuoles with a good yield (Fontes et al., 2010). Up to date, several vacuole or tonoplast proteome researches were applied on a few plants mainly on Arabidopsis thaliana, vacuoles or tonoplast were derived from mesophyll cells (Carter et al., 2004, Endler et al., 2006, Schulze, et al., 2012) or cell culture (Jaquinod et al 2006, Shimaoka et al 2004), cauliflower buds (Schmidt et al., 2007) and sugar beet taproots (Jung et al., 2015). Though the grape berry protoplasts and intact vacuoles were successfully isolated from Cabernet Sauvignon berry suspension-cultured cells (Fontes et al., 2010), the vacuoles isolated from grape berry or different development and ripening stages of grape berry mesocarp tissues were not achieved.
Project description:This microarray experiment provides data which shows of the effects on gene expression from the artificial application of certain hormones on their own or in combination on ripening berries from the Adelaide Hills Various hormones have been implicated as having an effect on the grape berry ripening process. Their application prior to ripening may change the gene expression profile at veraison. The identification of the genes which are expressed at different levels due to the various hormone treatments may give insight to key gene expression changes in the ripening process. Keywords: Hormone treatment-Development
Project description:In commercial fruit production, synchronized ripening and stable shelf life are important properties. The loosely clustered or non-bunching muscadine grape has unrealized potential as a disease resistant cash crop, but requires repeated hand harvesting due to its unsynchronized or long or heterogeneous maturation period. Genomic research can be used to identify the developmental and environmental factors that control fruit ripening and postharvest quality. This study coupled the morphological, biochemical, and genetic variations between ‘Carlos’ and ‘Noble’ muscadine grape cultivars with RNA-sequencing analysis during berry maturation. The levels of antioxidants, anthocyanins, and titratable acids varied between the two cultivars during the ripening process. We also identified new genes, pathways, and regulatory networks that modulated berry ripening in muscadine grape. These findings may help develop a large-scale database of the genetic factors of muscadine grape ripening and postharvest profiles and allow the discovery of the factors underlying the ripeness heterogeneity at harvest. These genetic resources may allow us to combine applied and basic research methods in breeding to improve table and wine grape ripening uniformity, quality, stress tolerance, and postharvest handling and storage.
Project description:Grape berry development is a highly coordinated, intricate and complex process with many morphological, biochemical and physiological changes occurring during the ripening process. Equally, ripening is an organoleptic characteristic linked to fruit development. The fruits Seedless (FS) and Victoria (VT) grape varieties exhibit many morphological and phytochemical differences, but genetic mechanisms underlying them remain poorly explored. Herein, we comparatively analysed the phenotypic and transcriptomic patterns of Victoria (VT) and Flame Seedless (FS) grape varieties during berry development. We studied the physiological analysis and transcriptomic profiles sequencing were performed at four berry developmental stages time-points (40, 50, 60 and 80 DPA). Notably, the VT variety berry size was comparatively larger to the FS variety. At maturity, 80DPA, the FS soluble solids were 61.8% higher than VT. Further, a total of 4889 and 2802 DEG’s were identified from VT and FS 40 DPA to 80 DPA development stages, respectively. 1386 DEGs were common in the two varieties. GO analysis identified Cysteine biosynthetic process, response to red light, chlorophyll binding, polysaccharide biosynthetic process and chloroplast thylakoid membrane as some of the dominant terms under the biological processes, molecular function and cellular component categories.