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Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

ABSTRACT: Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

PROVIDER: PRJEB41648 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4901800_1.fastq.gz	Fastqsanger.gz
	ERR4901800_2.fastq.gz	Fastqsanger.gz
	ERR4901801_1.fastq.gz	Fastqsanger.gz
	ERR4901801_2.fastq.gz	Fastqsanger.gz
	ERR4901802_1.fastq.gz	Fastqsanger.gz

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Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro

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RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro

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RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro

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2021-01-01 | E-MTAB-9836 | biostudies-arrayexpress

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Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro

Project description:Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro

| PRJEB41649 | ENA

RNAseq of murine MB49 tumors - WT and gal-9-KO - grown on C57BL/6 syngenic mice through serial transplantations

Project description:A number of studies have reported evidence of positive or negative contributions of galectin-9 (gal-9) to human and experimental malignancies. Some clinical observations and in vitro experiments suggest that cell-associated gal-9 has anti-metastatic effects. On the other hand, extra-cellular gal-9 consistently enhances tumor immune escape. So far, all animal studies on this subject have been focused on gal-9 released by infiltrating cells, without paying attention to gal-9 released by malignant cells. To address this issue, we derived by gene editing, isogenic clones - either positive or negative for gal-9 - from the MB49 murine bladder carcinoma cell line. A progressive reduction of tumor growth was observed when gal-9-KO cells were subjected to serial transplantations into syngenic mice but not into nude mice thus accounting the tumor growth reduction in syngenic mice to a better immune response. Tumor fragments were collected from WT and KO tumors at different steps of the experiment : 2nd growth cycle (WT = 5 samples ; KO = 6 samples ) ; 3rd growth cycle (WT = 5 samples ; KO = 7 samples) ; 4th growth cycle (WT = 4 samples ; KO = 3 samples), in order to study the differences between WT and KO tumors through the serial transplantations, by RNAseq analysis.

2020-07-01 | E-MTAB-9220 | biostudies-arrayexpress

RNAseq of murine MB49 cell line - WT and gal-9-KO - in vitro

Project description:RNAseq of murine MB49 cell line - WT and gal-9-KO - in vitro

| PRJEB40309 | ENA

Transcriptome profiling of wild-type (WT) and USP8 knockout (KO) CT26 cell lines

Project description:USP8 is one of DUBs and is frequently overexpressed or gain-of-function mutated in multiple types of human cancer. Importantly, USP8 has been identified as an immunomodulatory DUB as T cell-specific Usp8 deficiency disrupts regulatory T cell functions, leading to recruiting abundant CD8+ T cells in colons and resulting in the inflammatory bowel disease in mice. However, whether targeting USP8 can enhance anti-tumor immunity has not been reported. To further explore the physiological function of USP8, we performed the transcriptomic analysis to comprehensively understand which signaling pathways are mainly regulated by USP8 in cancer cells. Usp8 WT and KO CT26 cells were harvested for RNA-sequencing (RNA-seq).

2022-02-14 | GSE164558 | GEO

RNA sequencing of mouse CT26 knockout Pd-l1 cells rescued with WT or K262Q Pd-l1

Project description:Although membrane-anchored Pd-l1 has been well-studied for its engagement with PD-1 on T cells to evade anti-tumor immunity, whether Pd-l1 regulate oncogenic signaling pathways in tumor cells remains elusive. In this experiment, to further dissect roles of the K262 residue acetylation for Pd-l1 nuclear function, we profiled RNA expression of WT or K262Q mutant mouse Pd-l1 re-expressed in CT26 KO Pd-l1 cells. Methods: Total RNA fromCT26/Vector, CT26/WT or CT26 Pd-l1 KO cells was purified using Qiagen RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. Library preparation and sequenceing analysis were performed by BGI-Hong Kong Co. Ltd. Conclusions: Our study indicates that Pd-l1 acetylation modification may affect its function in nuclear.

2020-06-07 | GSE146557 | GEO

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