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RNA Sequencing of LT-HSCs and ST-HSCs from healthy adult human Bone Marrow

ABSTRACT: RNA Sequencing of LT-HSCs and ST-HSCs from healthy adult human Bone Marrow

PROVIDER: PRJEB41856 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4972860_1.fastq.gz	Fastqsanger.gz
	ERR4972860_2.fastq.gz	Fastqsanger.gz
	ERR4972861_1.fastq.gz	Fastqsanger.gz
	ERR4972861_2.fastq.gz	Fastqsanger.gz
	ERR4972862_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing of LT-HSCs and ST-HSCs from healthy adult human Bone Marrow

Project description:The transcriptome of LT-HSC (CD34+CD38-CD45RA+CD90+CD49f+) and ST-HSC (CD34+CD38-CD45RA+CD90-CD49f-) from healthy adult human Bone Marrow Cells were assessed by RNA-seq.

2022-04-06 | E-MTAB-9892 | biostudies-arrayexpress

Gene expression profiling of LT-HSCs and ST-HSCs conditionally expressing MLL-ENL

Project description:We have developed a new conditional transgenic mouse showing that MLL-ENL, at an endogenous-like expression level, induces leukemic transformation selectively in LT-HSCs. To investigate the molecular mechanism of leukemic transformation in LT-HSCs conditionally expressing MLL-ENL, we preliminarily performed comprehensive gene expression profiling of CreER-transduced LT-HSCs and ST-HSCs using cDNA microarray analysis. For initial screening of candidate genes invloved in the leukemic transformation, total RNA was extracted from colony-forming cells derived from LT-HSCs and ST-HSCs transduced with CreER or mock. Four samples were analyzed, and CreER-transduced LT/ST-HSC-derived cells were compared with mock-transduced LT/ST-HSC-derived cells, while CreER/mock-transduced LT-HSC-derived cells were compared with CreER/mock-transduced ST-HSC-derived cells.

2013-07-17 | E-GEOD-48539 | biostudies-arrayexpress

Gene expression profiling of LT-HSCs and ST-HSCs conditionally expressing MLL-ENL

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Single Cell RNA Sequencing of human bone marrow LT-HSCs.

Project description:10x single cell sequencing of LT-HSCs (CD34+CD38-CD90+CD45RA-CD49f+) from human bone marrow.

2022-04-06 | E-MTAB-9922 | biostudies-arrayexpress

RNA sequencing of eGFPlo and eGFPhi LT-HSCs in the bone marrow from Trim47+/eGFP mice

Project description:eGFPlo and eGFPhi LT-HSCs in the bone marrow from Trim47+/eGFP mice were isolated to explore the differentially expressed genes by RNA-seq.

2024-06-19 | GSE236767 | GEO

Single Cell RNA Sequencing of human bone marrow LT-HSCs.

Project description:Single Cell RNA Sequencing of human bone marrow LT-HSCs.

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Transcriptome data of bone marrow long-term hematopoietic stem cells (LT-HSCs) from mice with or without 3-month high cholesterol diet (HCD) treatment

Project description:The vulnerability of bone marrow hematopoiesis to perturbations of cholesterol metabolism is well documented, while the underlying cellular and molecular mechanisms remain poorly understood. Here we reveal a distinct cholesterol metabolic signature of hematopoietic stem cells (HSCs) within the hematopoietic compartment.To identify the phenotype switching and function variation in BM LT-HSCs with HCD treatment, we performed RNA-seq of LT-HSCs from the bone marrow of mice with or without3-month HCD treatment.

2023-03-29 | GSE214035 | GEO

Transcriptome data of bone marrow long-term hematopoietic stem cells (LT-HSCs) from mice with or without 3-month serine/glycine-free diet (SGFD) treatment.

Project description:The vulnerability of bone marrow hematopoiesis to perturbations of serine metabolism as well as the underlying cellular and molecular mechanisms remain poorly understood. Here we reveal a distinct serine metabolic signature of hematopoietic stem cells (HSCs) within the hematopoietic compartment.To identify the phenotype switching and function variation in BM LT-HSCs with SGFD treatment, we performed RNA-seq of LT-HSCs from the bone marrow of mice with or without3-month SGFD treatment.

2024-08-17 | GSE249609 | GEO

Expression data from LT-HSCs from bone marrow non-irradiated, 8Gy CDR and 8Gy FLASH total body irradiated mice

Project description:Total body irradiation (TBI) of mice using two dose rates, conventional dose rate (CDR) versus flash dose rate (FLASH), induced transient decrease of number of LT-HScs in bone marrow and a total recovery of these cells 15 days after TBI We used microarrays to detail the global programme of gene expression underlying the recovery of LT-HSCs and identified distinct classes of up or down- regulated genes according the modality of irradiation

2015-01-01 | GSE54526 | GEO

Loss of Phf6 prevents aging associated transcriptomic profile changes in LT-HSCs

Project description:Loss of Phf6 prevents the functional decline and immunophenotypic changes associated with age-related, long-term repopulating hematopoietic stem cell (LT-HSC) exhaustion. To identify the underlying molecular mechanisms that account for these differences, we performed RNA-seq profiling of LT-HSCs isolated from the bone marrow of Phf6 wild-type and knock-out, young (16-week-old) and aged (24-month-old) C57BL/6 mice. Our analysis revealed that LT-HSCs isolated from 24-month-old, Phf6 knockout mice retained the molecular signatures associated with young LT-HSCs whereas LT-HSCs isolated from aged, Phf6 wild-type mice acquired signatures consistent with HSC exhaustion. Mechanistically, these data revealed important roles for key metabolic pathways including glutathione metabolism and sterol biosynthesis, as well as cell-cell interaction and signaling pathways such as the interferon and TGF-beta responses.

2022-11-01 | GSE165695 | GEO

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