Project description:Chemoautotrophic bacteria from the SUP05 clade often dominate anoxic waters in marine oxygen minimum zones (OMZs) where reduced sulfur can fuel carbon fixation and denitrification. Some members of the SUP05 clade are facultative aerobes that thrive at the boundaries of OMZs where they experience fluctuations in dissolved oxygen (DO). The degree to which SUP05 contribute to nitrate reduction in these regions depends on their sensitivity to oxygen. We evaluated growth and quantified differences in gene expression in Ca. T. autotrophicus strain EF1 from the SUP05 clade under high DO (22 μM), anoxic, and low DO (3.8 μM) concentrations. We show that strain EF1 cells respire oxygen and nitrate and that cells have higher growth rates, express more genes, and fix more carbon when oxygen becomes available for aerobic respiration. Evidence that facultatively aerobic SUP05 are more active and respire nitrate when oxygen becomes available at low concentrations suggests that they are an important source of nitrite across marine OMZ boundary layers.
2020-06-01 | GSE144480 | GEO
Project description:Three MAGs of SUP05 cluster (family Thioglobaceae) from deep-sea non-buoyant hydrothermal plumes
Project description:In order to study the effect of Ncx1 deletion on the HSC-supportive microenviromrent in the AGM niche, caudal parts from E9.5 (20-23 sp) Ncx1-/- and Ncx1+/+ littermate controls (4 embryos/group) were dissected and processed into single cell suspensions and dead/damaged cells were removed by magnetic separation. Resulting cell suspension from Ncx1-/- and Ncx1+/+ embryos loaded onto independent channels of a Chromium chip before single cell partitioning and barcoding on Chromium Controller (10x Genomics).