Project description:Dendritic cells increase expression of pro-inflammatory cytokines and chemokines and undergo metabolic reprogramming upon TLR activation. Mice fed conventional diet (CD) or 60% high-fat diet (HFD) were treated by skin application of imiquimod (IMQ) during 6 days and classical dendritic cells (cDC) were isolated from inguinal lymph nodes (iLN) to reveal inflammatory and metabolic pathways that are sensitive to TLR7/8 activation and a high fatty acid environment. We used microarray to identify pathways in classical dendritic cells from IMQ-treated mice that link inflammation and metabolism in a high fatty acid environment.
Project description:A gene expression profile of the conventional dendritic cell lineage 1 from murine mesenteric lymph nodes (MLN) were constructed based on RNA-seq from nine biological biopsies. The gene expression profile was afterwards used to infer (in silico) a cDC1-specific protein-protein interactome.
Project description:We combined scRNA-seq and a photoconvertible mouse model to study the spatiotemporal dynamics of macrophages and dendritic cells (DCs) in murine tumours including their migration to the lymph node, including the effects of immune checkpoint therapy (anti-PD-L1).
Project description:Topical (epicutaneous, e.c.) application of the adjuvant CpG ODN during immunization leads to a robust immune response compared to when subcutaneous (s.c.) administration. Dendritic cells are hematopoietically derived cells that are important in cross-presenting to and activating CD8 T cells. Dermal dendritic cells are one of the two major dendritic cell subsets found in the skin which mobilize from the skin to draining lymph nodes to present to T cells upon activation. Dermal dendritic cells are found in skin draining lymph nodes around 24 hours post immunization. To determine how the immune system respond differently between e.c. versus s.c. administration of CpG ODN, we evaluated changes in the skin draining lymph node environment upon the two routes of adjuvant application. Expression chemokines and chemokine receptors were assessed with real-time qPCR. To determine the changes in the skin draining lymph node environment (cytokine and cytokine receptor levels) upon immunization via real time RT-PCR.