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RNA-seq of oocytes and zygotes from pabpn1l ko and wt female mice

ABSTRACT: RNA-seq of oocytes and zygotes from pabpn1l ko and wt female mice

PROVIDER: PRJEB43700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR5488690_1.fastq.gz	Fastqsanger.gz
	ERR5488690_2.fastq.gz	Fastqsanger.gz
	ERR5488691_1.fastq.gz	Fastqsanger.gz
	ERR5488691_2.fastq.gz	Fastqsanger.gz
	ERR5488692_1.fastq.gz	Fastqsanger.gz

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RNA-seq of oocytes and zygotes from pabpn1l ko and wt female mice

Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.We collect samples of each period from pabpn1l ko and wt female mice during different stages. Each group contained a total of 30 oocytes or zygotes. In the initial step, we spiked 2 × 106 mRNA-EGFP, transcribed in vitro, into each group.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice seperately.

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Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.So,we collect samples of each period from pabpn1l ko and wt female mice during different stages.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice separately.

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RNA-seq of pabpn1l ko and wt female mice oocytes and zygotes

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Project description:After the library was qualified, the library was pooled according to the effective concentration and the demand of target offline data, and was sequenced by Illumina platform. Since PABPN1L can bind the poly(A) tail, we propose that it participates in the process of post-transcriptional regulation and degradation of maternal mRNA. We investigated the mRNA changes in GV oocytes, MII oocytes, and fertilized eggs using RNA-seq. GV oocytes, MII oocytes and zygotes were collected and mixed from three mice of each genotypes separately.

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