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Comparing the Transcriptomes of WT oocytes with Lsm14b KO oocytes by RNA-Seq Analysis

ABSTRACT: Comparing the Transcriptomes of WT oocytes with Lsm14b KO oocytes by RNA-Seq Analysis

PROVIDER: PRJNA842232 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19393853_1.fastq.gz	Fastqsanger.gz
	SRR19393853_2.fastq.gz	Fastqsanger.gz
	SRR19393854_1.fastq.gz	Fastqsanger.gz
	SRR19393854_2.fastq.gz	Fastqsanger.gz
	SRR19393855_1.fastq.gz	Fastqsanger.gz

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Comparing the Transcriptomes of WT oocytes with Lsm14b KO oocytes by RNA-Seq Analysis

Project description:The success of human reproduction relies on high quality oocytes. Oocyte quality is manifested by the competence to complete meiosis, to be fertilized, and to support embryonic development. This meiotic and developmental competence is gradually established during the course of oocyte and follicle development, and is determined in large part by the autonomous gene expression program intrinsic to the oocyte. In order to explore the regulatory role of LSM14B in oocyte, we analyzed the effect of Lsm14b KO on gene expression in GV-stage fully-grown oocytes (FGOs) in mice by comparing the corresponding transcriptomes via RNA-Seq Analysis.

2023-09-08 | GSE204773 | GEO

Identifying the Translatome of WT and Lsm14b KO GV-Stage Oocytes in mice via Scarce Sample Polysome Profiling (SSP-profiling)

Project description:RNA-binding proteins (RBPs) have essential functions during oocyte development.In order to explore the regulatory role of RNA-binding protein LSM14B in oocyte, we Identified the translatome of WT and Lsm14b KO GV-Stage Oocytes in mice via Scarce Sample Polysome Profiling (SSP-profiling).

2023-09-08 | GSE206270 | GEO

Mus_Oocyte_LC-MS/MS-mRBPome capture of oocytes identifies the RNA-binding protein LSM14B, an essential regulator of meiosis in mouse oocytes

Project description:A quantitative mass-spectrometry-based proteome profiles of oocytes in WT and Lsm14b KO mouse

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Loss of LSM14b affects the meiosis process of mouse oocytes

Project description:RNA-Seq can comprehensively and quickly obtain almost all transcripts and information of specific cells in rustic states.Here,we compared up-regulated genes in GV and MII oocytes from LSM14b deficient mice and wild-type mice by RNA-Seq,highlighting the important role of LSM14b in meiosis of mouse oocytes.

2023-12-01 | GSE198414 | GEO

LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

Project description:Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria associated ribonucleoprotein domain (MARDO). But the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4, and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, couple the MARDO with mRNA clearance and oocyte meiotic maturation.

2023-10-15 | GSE231648 | GEO

Loss of LSM14b affects the meiosis process of mouse oocytes

Project description:Loss of LSM14b affects the meiosis process of mouse oocytes

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Identifying the Translatome of WT and Lsm14b KO GV-Stage Oocytes in mice via Scarce Sample Polysome Profiling (SSP-profiling)

Project description:Identifying the Translatome of WT and Lsm14b KO GV-Stage Oocytes in mice via Scarce Sample Polysome Profiling (SSP-profiling)

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LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

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RNA-seq of oocytes and zygotes from pabpn1l ko and wt female mice

Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.We collect samples of each period from pabpn1l ko and wt female mice during different stages. Each group contained a total of 30 oocytes or zygotes. In the initial step, we spiked 2 × 106 mRNA-EGFP, transcribed in vitro, into each group.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice seperately.

2021-03-26 | E-MTAB-10233 | biostudies-arrayexpress

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Project description:We analysed 3~4 repeats of 3 groups of mouse MII oocytes including 2-month-old WT, 2-month-old Tet2-KO, 11-month-old WT to find Tet2 function on female mice fertility.

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