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Serratia marcescens mutants

ABSTRACT: The effect of secondary metabolites produced by Serratia marcescens on Aedes aegypti and its microbiota

PROVIDER: PRJEB45268 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR5976320_1.fastq.gz	Fastqsanger.gz
	ERR5976320_2.fastq.gz	Fastqsanger.gz
	ERR5976321_1.fastq.gz	Fastqsanger.gz
	ERR5976321_2.fastq.gz	Fastqsanger.gz
	ERR5976322_1.fastq.gz	Fastqsanger.gz

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Transcriptional impact of eepR mutation in Serratia marcescens

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Project description:In order to identify changes in the global mRNA transcriptome caused by deletion of the RNA-binding protein Hfq in Serratia marcescens, total mRNA was isolated from wild type Serratia marcescens Db10 and an otherwise isogenic strain carrying an in-frame deletion of the hfq gene (SMDB11_4482) and analysed by RNAseq. Four independent biological replicates were sequenced for each strain using the Illumina HiSeq platform. The data was used to identify the nature and extent of changes in transcript level between the two strains and to inform on the role of Hfq in virulence of Serratia marcescens, an opportunist bacterial pathogen.

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Insight into the regulation of prodigiosin production by temperature in Serratia marcescens through transcriptome analysis

Project description:We report the application of transcriptome sequencing technology for high-throughput profiling of Serratia marcescens for producing prodigiosin. By obtaining over 163 million bases of sequence from Serratia marcescens genome DNA, we generated transcriptome -state maps of Serratia marcescens 12h cells, 24h cells, and 36h cells at 30C and 37C,respectively. We explored the mechanism of S. marcescens response temperature regulation at the transcription level through transcriptome sequencing technology. We found that the pig gene cluster at low temperature would favor at the transcriptional level, however, higher temperature resulting in instability and loss of enzyme activity. Numerous amino acid metabolic pathways involved in prodigiosin biosynthesis in S. marcescens responded to temperature changes, and metabolic fluxes were directed towards prodigiosin biosynthesis. At the same time, quorum sensing, two-component regulatory system and sRNA were stimulated by temperature to regulate PG biosynthesis and involve strain virulence and exclusive genes. Moreover, inhibition factors was the one reason for S. marcescens incapable synthesis of prodigiosin at 37C. This study laid a good foundation for understanding the biological functions of prodigiosin, improving the temperature tolerance of industrial strains, and excavating temperature-sensitive regulatory elements.

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