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IiCLIP protocol benchmarking with PTBP1

ABSTRACT: iiCLIP protocol benchmarking with PTBP1

PROVIDER: PRJEB47097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR6544650.fastq.gz	Fastqsanger.gz
	ERR6544651.fastq.gz	Fastqsanger.gz
	ERR6544647.fastq.gz	Fastqsanger.gz
	ERR6544648.fastq.gz	Fastqsanger.gz
	ERR6544649.fastq.gz	Fastqsanger.gz

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iiCLIP protocol benchmarking with PTBP1

Project description:We describe an improved individual nucleotide resolution CLIP protocol (iiCLIP), which can be completed within 4 days from UV crosslinking to libraries for sequencing. For benchmarking, we directly compared PTBP1 iiCLIP libraries with the iCLIP2 protocol produced under standardised conditions with 1 million HEK293 cells, and with public eCLIP and iCLIP PTBP1 data. There are 3 PTBP1 iiCLIP libraries, 1 input iiCLIP library and 1 PTBP1 iCLIP2 library produced in this study.

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