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Understanding the role of mycobacteriophage D29 stoperators in promoting transcription

ABSTRACT: A CRISPRi based investigation reveals that multiple promoter elements drive gene expression from the genome of mycobacteriophage D29.

PROVIDER: PRJEB48008 | ENA |

REPOSITORIES: ENA

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Promoter activity associated with Mycobacteriophage D29 stoperators

Project description:A CRISPRi based investigation reveals that multiple promoter elements drive gene expression from the genome of mycobacteriophage D29.

| PRJEB48007 | ENA

O-Glycosylation of Mycobacteriophage Proteins

Project description:Provided herein is the first evidence that mycobacteriophage, indeed phage of any kind, have surface proteins (capsid and/or tail tube proteins) that are O-glycosylated. The glycosyltranferases that glycosylate these proteins are encoded in the phage genomes. In addition, for the three mycobacteriophages invested in this study, the glycans appear to be attached exclusively at a C-terminal serine residue. Each phage expresses a unique and novel O-glycan. The LC-MS files deposited here are the preliminary evidence that these mycobacteriophage proteins are glycosylated. A follow-up investigation will examine the composition and structure of the O-glycans in more detail.

2024-08-10 | PXD041690 | Pride

Transcriptomic profile of Mycobacteriophage Giles

Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen. Total RNA was isolated, depleted of rRNA and prepped for sequencing (Illumina) during early and late infection of Giles and a lysogen.

2013-04-23 | E-GEOD-43434 | biostudies-arrayexpress

Transcriptomic profile of Mycobacteriophage Giles

Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen.

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Transcriptomic profile of Mycobacteriophage Adephagia

Project description:The gene expression during early (30min) and late (210 minutes) infection of Mycobacteriophage Adephagia was analyzed, as well as in a Adephagia lysogen.

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Transcriptomic profile of Wildcat mycobacteriophage during lytic growth.

Project description:The goal of this sequencing project was to measure transcription levels of Wildcat mycobacteriophage during lytic growth.

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Transcriptomic profiles of D29, StarStuff and L5

Project description:The gene expression profiles of mycobacteriophage D29 and StarStuff were analyzed 15min, 30min, 60min and 150min post-infection (MOI=3), as well as a StarStuff lysogen. The gene expression profiles of 30min and 150min post-induction of an L5 thermo-inducible lysogen, as well as the L5 lysogen itself.

2017-11-13 | GSE99182 | GEO

Transcriptomic profile of ZoeJ mycobacteriophage during various stages of growth.

Project description:The goal of this sequencing project was to measure transcription levels of ZoeJ mycobacteriophage during different stages of the lifecycle, including lysogeny.

2019-02-18 | GSE124840 | GEO

RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage

Project description:We present transcriptomic analysis of an infection by a cluster A4 mycobacteriophage of its host, Mycobacterium smegmatis, at six time points (n = 2). Viral transcription is detectable 5 minutes into infection, and viral mRNA makes up the vast majority of all transcription by 30 minutes. Kampy, like many phages, has a genome organized into two arms bearing early- and late-phase genes. We identify two transcriptional start sites (TSS’s) consistent with an early promoter driving expression of half of the genome, along with a second, late-stage promoter that becomes active around 30 minutes after the start of infection.

2015-02-02 | GSE60592 | GEO

Transcriptomic profile of Mycobacteriophage Adephagia

Project description:Transcriptomic profile of Mycobacteriophage Adephagia

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