Project description:Human tumour cell lines PC3, DU145, U87 and U373 (prostate carcinoma and glioblastoma) were treated with photosensitizers 5-aminolaevulinic acid (5-ALA) or photofrin. Then, the cells were irradiated sublethally with 635 nm laser light.<br>After photodynamic therapy, the cells were grown at 37°C for 4 or 24 hours in the dark until extraction of total RNA and expression profiling.
Project description:Murine prostate carcinoma cell lines TRAMP-C1 and TRAMP-C2 were grown for 16 h at 37M-0C with photosensitizer 5-aminolaevulinic acid (5-ALA; 50 M-5g/ml). Then, the cells were sublethally irradiated with laser light (635 nm) at room temperature (using RPMI1640 without phenol red as medium). After photodynamic therapy, the cells were cultivated at 37M-0C for 4 h and 24 h, respectively. After that time total RNA was extracted for expression profiling.
Project description:Differentiation therapy with all-trans retinoic acid (ATRA) is well established for acute promyelocytic leukemia (APL). However, the narrow application and tolerance development of ATRA remain to be improved. In this study, we challenged glycosylation inhibitor to obtain better efficiency than ATRA alone. As a result, we found that the combination of fucosylation inhibitor 6-alkynylfucose (6AF) with ATRA have profound effect for differentiation, shown by expression changes of differentiation markers CD11b, CD11c, with significant morphological change in NB4 and HL-60 cells. From lectin blot assay, we found that ATRA or 6AF alone could decrease core fucosylation, the combination of these two agents efficiently decreases the expression of core fucosylation. To reveal molecular mechanisms to reveal 6AF effect for ATRA induced differentiation, we next performed microarray analysis using NB4 cells. From pathway analysis using DAVID software, we found that C-type lectin receptor (CLR) signaling pathway was enriched as high significance. From real time PCR analysis, using NB4 and HL-60 cells, FcRI, CLEC6A, CASP1, IL-1, EGR2/3, the components of CLR, and Akt, were indeed upregulated by 6AF in ATRA induced differentiation. These suggest that the involvement of CLR signaling pathway in 6AF effect of ATRA induced differentiation.
Project description:Analysis of photodynamic therapy with hypericin of nasopharyngeal carcinoma cells CNE-2 in different time-points at gene expression level. The hypothesis tested in the present study was that HY-PDT could induce apoptosis on CNE-2 cells via intrinsic pathways. Results provide important information of the response of apoptosis, such as specific oxidative stress genes, mitochondrial related genes, cell cycle related genes, etc.
Project description:Analysis of photodynamic therapy with hypericin of nasopharyngeal carcinoma cells CNE-2 in different time-points at gene expression level. The hypothesis tested in the present study was that HY-PDT could induce apoptosis on CNE-2 cells via intrinsic pathways. Results provide important information of the response of apoptosis, such as specific oxidative stress genes, mitochondrial related genes, cell cycle related genes, etc. Total RNAs were isolated from the cultured cells treatment with 20 μg/ml HY and irradiation then cultured in dark for 0, 2, 6, 12, 20 h respectively compared to cells treatment with 20 μg/ml HY only then cultured in dark for 20 h.
Project description:The efficacy of photodynamic therapy for treating premalignant and malignant tumors is often limited by the emerging resistant tumor cells. We have developed experimental model systems to study the mechanisms associated with resistance to photodynamic therapy induced by structurally similar photosensitizers (two novel porphyrin-based photosensitizers and temoporfin) in mouse mammary carcinoma cell line 4T1. Photodynamic therapy resistant clones were obtained in vitro by exposure to constant photosensitizer concentration and irradiation with increasing light doses.
