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Gene expression profiling of Trop2+ cells in mouse Apc-deficient small intestinal hyperplastic epithelium

ABSTRACT: Gene expression profiling of Trop2+ cells in mouse Apc-deficient small intestinal hyperplastic epithelium

PROVIDER: PRJEB50466 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR8138090.fastq.gz	Fastqsanger.gz
	ERR8138091.fastq.gz	Fastqsanger.gz
	ERR8138092.fastq.gz	Fastqsanger.gz
	ERR8138083.fastq.gz	Fastqsanger.gz
	ERR8138084.fastq.gz	Fastqsanger.gz

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Gene expression profiling of Trop2+ cells in mouse Apc-deficient small intestinal hyperplastic epithelium

Project description:Tumor Associated Calcium Signal Transducer 2 (TACSTD2) is one of the cancer-related genes whose overexpression correlates with tumor progression and invasiveness in human colorectal cancer. TACSTD2 gene encodes for a transmembrane glycoprotein TROP2, which is implicated in altered expression of epithelial-mesenchymal transition (EMT) markers and may play a role in metastasis formation. To monitor the onset of TROP2 in hyperplastic cells and its effect on aberrant signaling potentially leading to tumor growth, we isolated epithelial cells from the mouse small intestine 7 days after disruption of the Adenomatous polyposis coli (Apc) gene. Loss of APC tumor suppressor function is the most common initial step in the development of colon cancer in humans. In the mice, the epithelial hyperproliferation occurs within a few days after Apc disruption, accompanied by the formation of ectopic crypts, followed by the formation of microadenomas over time. We performed expression profiling of Trop2+ and Trop2- hyperproliferating cells and, in addition, non-proliferating cells of the intestinal epithelium, including predominantly differentiated cells.

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Project description:The first step in the development of human colorectal cancer is the aberrant hyperactivation of the Wnt signaling pathway, predominantly caused by inactivating mutations in the adenomatous polyposis coli (Apc) gene encoding an essential tumor suppressor. The gene encoding transcriptional factor msh homeobox 1 (Msx1) displayed robust upregulation upon Apc inactivation in intestinal epithelium isolated in mice harboring the conditional allele of the Apc gene. To identify the gene signature in the small intestine upon Msx1 depletion, small intestinal epithelium from mice harboring conditional alleles of Apc and Msx1 was isolated and the gene expression profile was compared with control mice harboring the conditional allele of Apc only.

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