BACTERIAL COMMUNITY FROM AN ANAEROBIC FLUIDIZED BED REACTOR INVOLVED IN THE LINEAR ALCOHOL ETHOXYLATE DEGRADATION
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ABSTRACT: Through the pyrosequencing analysis of samples from support mateial and phase separator from the anaerobic fluidized bed reactor total of 83 genera was found, which 18 are involved to the nonionic surfactant degradation.
Project description:This paper presents the numerical investigation of the effects of the general bed characteristics such as superficial gas velocities, bed temperature, bed heights and particle size, on the direct synthesis in a 3D fluidized bed reactor. A 3D model for the gas flow, heat transfer, and mass transfer was coupled to the direct synthesis reaction mechanism verified in the literature. The model was verified by comparing the simulated reaction rate and dimethyldichlorosilane (M2) selectivity with the experimental data in the open literature and real production data. Computed results indicate that superficial gas velocities, bed temperature, bed heights, and particle size have vital effect on the reaction rates and/or M2 selectivity.
Project description:An industrial fluidized bed reactor was designed to convert an aqueous solid laden stream into a consistent granular product. CFD simulations were run using the MFiX two-fluid model for a fluidizing bed operating at 650?°C. A set of simulations were run over a Latin-hypercube sample of five model parameters - bed particle size, bed particle density, coal particle size, spray feed flow rate, and fluidizing gas flow rate. Data from the simulations were collected on three quantities of interest - bed differential temperature, low solids velocity, and bed void fraction. The data presented here is the full set of response surfaces generated using the process Gaussian response surface model in the Dakota toolkit, as well as the table of data for coefficients of the fitted model. The fits to the five-dimensional Gaussian Process models were 0.7797, 0.8664, and 0.9440 for the temperature, velocity, and solids packing, respectively.
Project description:The current paper presents research on bio-oil production from Tung seed residues fed at 500 g/h via fast pyrolysis in a fluidized-bed. The objective was to investigate the influence of temperature on bio-oil production in a pyrolysis process. Three portions Tung residues were studied, Tung seed outer shells (TO), Tung seed inner shells (TI), and pressed residues of oil seeds (RS), all having particle sizes of 0.150-0.500 mm. The process temperatures were 350-500 °C. The physical and chemical properties of pressed residue particles were characterized by ASTM standard methods. Bio-oil component identification was done using GC-MS. Experimentally derived data showed an optimal pyrolysis temperatures for all three types of Tung residues (TO, TI and RS) of 400 °C, yielding respective maximum bio-oil yields of 53.46, 52.81, and 62.85 wt% on a dry basis (db). Apart from having highest bio-oil yield, RS produced bio-oil with the highest carbon content, leading to its greatest lower heating value (LHV), 28.05 MJ/kg (db). The main bio-oil components were acids, nitrogen compounds, and hydrocarbons. Char yield was reduced with increased temperature. Tung seed outer shells produced the highest char level (39.26 wt%) while RS gave highest char quality in term of density and heating value.
Project description:Genome-wide epigenetic changes such as histone modifications form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has made to decrease the input required for gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by next generation sequencing (i.e. ChIP-seq) to allow scarce primary tissues of specific type from patients and lab animals to be tested. However, there has been very little effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (Low-Input Fluidized-bed Enabled Chromatin Immunoprecipitation combined with sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine.
Project description:The capacity of electroactive bacteria to exchange electrons with electroconductive materials has been explored during the last two decades as part of a new field called electromicrobiology. Such microbial metabolism has been validated to enhance the bioremediation of wastewater pollutants. In contrast with standard materials like rods, plates, or felts made of graphite, we have explored the use of an alternative strategy using a fluid-like electrode as part of a microbial electrochemical fluidized bed reactor (ME-FBR). After verifying the low adsorption capacity of the pharmaceutical pollutants on the fluid-bed electrode [7.92 ± 0.05% carbamazepine (CBZ) and 9.42 ± 0.09% sulfamethoxazole (SMX)], our system showed a remarkable capacity to outperform classical solutions for removing pollutants (more than 80%) from the pharmaceutical industry like CBZ and SMX. Moreover, the ME-FBR performance revealed the impact of selecting an anode potential by efficiently removing both pollutants at + 200 mV. The high TOC removal efficiency also demonstrated that electrostimulation of electroactive bacteria in ME-FBR could overcome the expected microbial inhibition due to the presence of CBZ and SMX. Cyclic voltammograms revealed the successful electron transfer between microbial biofilm and the fluid-like electrode bed throughout the polarization tests. Finally, Vibrio fischeri-based ecotoxicity showed a 70% reduction after treating wastewater with a fluid-like anode (+ 400 mV), revealing the promising performance of this bioelectrochemical approach.
Project description:This work established a three-dimensional model of a chemical looping system with multistage reactors coupled with hydrodynamics and chemical reactions. The thermal characteristics in the chemical looping combustion (CLC) system were simulated using coal as fuel and hematite as an oxygen carrier (OC). Some significant aspects, including gas composition, particle residence time, backmixing rate, wall erosion, carbon capture rate, etc., were investigated in the simulation. Owing to the optimization by adding baffles in the fuel reactor (FR), the gas conversion capacity of the multistage FR was high, where the outlet CO2 concentration was as high as 93.8% and the oxygen demand was as low as 3.8%. Through tracing and analyzing the path of char particles, we found that the residence time of most char particles was too short to be fully gasified. The residual char will be entrained into the air reactor (AR), reducing the CO2 capture rate, which is only 80.3%. In the simulation, the wall erosion on the cyclone could be relieved by increasing the height of the horizontal pipe. In addition, improving the structure of the AR loop seal could control the residual char entrained by OC particles to the AR, and the CO2 capture rate was increased up to 90% in the multistage CLC reactor.