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Transcriptome analysis of wandering larval hemocytes in Toll10b and gcm background in Drosophila

ABSTRACT: Transcriptome analysis of wandering larval hemocytes in Toll10b and gcm background in Drosophila

PROVIDER: PRJEB54818 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9969640.fastq.gz	Fastqsanger.gz
	ERR9969641.fastq.gz	Fastqsanger.gz
	ERR9969633.fastq.gz	Fastqsanger.gz
	ERR9969634.fastq.gz	Fastqsanger.gz
	ERR9969635.fastq.gz	Fastqsanger.gz

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1 - 5 of 9

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Transcriptome analysis of wandering larval hemocytes in Toll10b and gcm background in Drosophila

Project description:The aim of the experiment is to establish the impact of the interaction between the hemocyte differentiation factor gcm and the Toll pathway in the immune cells of the Drosophila larva. The hemocytes were collected from 3rd instar larvae of the following genotypes: Toll10B/+, gcm26/+ and gcm26/+;Toll10B/+.

2023-06-09 | E-MTAB-11970 | biostudies-arrayexpress

Sequencing of the transcriptome from embryonic and larval hemocytes in gcm knock down background

Project description:Hemocytes were sorted from stage 16 embryos and wandering 3rd instar larvae in which gcm was knocked down using gcmRNAi and the driver srp(hemo)Gal4. The experiments were done in triplicate. The wild type controls at stage 16 embryo and wandering larvae are available under this assession number: E-MTAB-8702.

2022-10-18 | E-MTAB-11966 | biostudies-arrayexpress

Sequencing of the transcriptome from embryonic and larval hemocytes in gcm knock down background

Project description:Sequencing of the transcriptome from embryonic and larval hemocytes in gcm knock down background

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Single cell RNA on hemocytes from wandering larvae of Drosophila melanogaster not infested and infested by Leptopilina boulardi

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RNA-seq of Drosophila melanogaster neurons, glia and hemocytes at embryonic stage 16 and third instar wandering larvae

Project description:We produced bulk RNA sequencing data on Drosophila neurons, glia and hemocytes at embryonic and larval stages to extrapolate the most significant and specific features that identify a cell type as a whole. We also tracked changes in the expression in the two stages. We identified stable features that define a cell type functionally and developmentally as well as plastic features including stage-specific metabolic states, which likely depend on the environment.

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Bulk RNAseq on hemocytes from stage 16 embryos and wandering 3rd instar larvae of Drosophila melanogaster

Project description:The aim of this experiment is to compare the transcriptome of Drosophila hemocytes at two developmental stages: in stage 16 embryo, mostly free from any immune challenge and in larvae, which grow in bacteria rich environment. Transgenes inducing the expression of RFP in the hemocytes were used to sort the hemocytes from the two stages before carrying out the sequencing.

2020-03-12 | E-MTAB-8702 | biostudies-arrayexpress

Bulk RNAseq on hemocytes from stage 16 embryos and wandering 3rd instar larvae of Drosophila melanogaster

Project description:Bulk RNAseq on hemocytes from stage 16 embryos and wandering 3rd instar larvae of Drosophila melanogaster

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Project description:We used RNA-seq in a derived European Drosophila melanogaster population from Germany (MU) to examine coding gene expression variation in the larval fat body during the late wandering third instar stage.

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Expression profile of third instar larval fat body and midgut tissues

Project description:We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

2010-11-02 | E-GEOD-25025 | biostudies-arrayexpress

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