Project description:This experiment was performed to examine transcriptional differences between WT and Tgfbr2 KO antigen-specific CD8 cells activated in established chronic infection.
Project description:This experiment was performed to examine transcriptional differences between differentiated (Tim3+) and stem-like (CXCR5+) WT vs. Tgfbr2 KO antigen-specific CD8 cells.
Project description:This experiment was performed to examine transcriptional differences between differentiated (Tim3+) and stem-like (CXCR5+) WT vs. Tgfbr2 KO antigen-specific CD8 cells at day 30 after LCMV clone 13 infection.
Project description:Single-cell RNA-seq in mice with subcutaneous tumors was used to determine if TGFβR2 signaling in DPT+ universal fibroblasts drives LRRC15+ myofibroblast differentiation using DptIresCreERT2Tgfbr2fl/fl and WT control mice.
Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.
Project description:Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. We found that Hgfac KO mice showed delayed wound healing in an endoscopic wound model. To dissect the celluar and molecular mechanisms of tissue healing, we collected tissues from day2 wounds or intact tissues from Hgfac WT and KO mice using a skin biopsy punch and dissociated cells for scRNA-seq analysis.
Project description:To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.