Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA-seq of stromal cells in WT and LRRC15 DTR mice

ABSTRACT: ScRNA-seq of stromal cells in WT and LRRC15 DTR mice

PROVIDER: PRJEB55257 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR10037225_1.fastq.gz	Fastqsanger.gz
	ERR10037225_2.fastq.gz	Fastqsanger.gz
	ERR10037226_1.fastq.gz	Fastqsanger.gz
	ERR10037226_2.fastq.gz	Fastqsanger.gz
	ERR10037227_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

ScRNA-seq of stromal cells in WT and LRRC15 DTR mice

Project description:KPR tumors were subcutaneously implanted in Lrrc15DTRGFPwt/wt (DTR–) or Lrrc15DTRGFPwt/ki (DTR+) mice and DT treatment was initiated in both DTR– and DTR+ mice when tumors reached a mean volume of 100-200mm3. scRNAseq of CD24-CD45- stromal cells from a total of 40 tumor-bearing animals across both DTR– and DTR+ genotypes was carried out at four different time points including 10 days post tumor implantation and immediately prior to initiation of DT treatment (IOT) (referred to as day 0) and on days 7, 14, and 21 post IOT.

2022-08-17 | E-MTAB-12036 | biostudies-arrayexpress

ScRNA-seq of stromal and immune cells in WT and TGFBR2 KO mice

Project description:ScRNA-seq of stromal and immune cells in WT and TGFBR2 KO mice

| PRJEB55182 | ENA

Streptococcus pneumoniae membrane proteomics of WT and scRNA mutants

Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.

2024-08-19 | PXD054463 | Pride

scRNA-seq of lung stromal cells (CD45-CD31-CD326-) from C57BL/6J mice

Project description:To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.

2022-07-19 | E-MTAB-11879 | biostudies-arrayexpress

Expression data from FITC challenged skin of CLEC4C-DTR mice vs. littermate controls treated with diptheria toxin

Project description:CLEC4C-DTR exhibit exacerbated contact hypersensitivty to the FITC hapten. Here we assess the transcriptional differences in whole skin of FITC challenged CLEC4C-DTR+ mice and DTR- mice treated with diptheria toxin. Overall, CLEC4C-DTR upregualated 439 genes and downregulated 152 genes compared to transgene negative controls, filtering output on 2 fold expression level difference and FDR<0.05.

2020-10-28 | GSE160261 | GEO

scRNA-seq analysis of dendritic cells in skin draining lymph nodes of CLEC4C-DTR mice treated with FITC hapten

Project description:Skin draining lymph node dendritic cell populations are known to be diverse and distinguishable via their migratory or resident properties as well as by lineage. We analyzed these populations at steady state and during the sensitization phase of the Th2-driven FITC-hapten contact hypersensitivity (CHS) model. We also analyzed the dendritic cells populations of CLEC4C-DTR+ mice treated with diptheria toxin, which demonstrate exacerbated pathology in the FITC-CHS model. We demonstrate the diversity of dendritic cell populations at steady state and during type 2 inflammation, and surprisingly, reveal a skewing of the DC populations in DT treated CLEC4C-DTR+ mice, with an enrichment of DC2 migratory populations and depletion of CD326+ migratory cells in addition to pDC.

2020-12-31 | GSE161604 | GEO

Expression profiles of satellite cells and alpha7Sca1 cells in mdxITGAM-DTR mice

Project description:We took advantage of a dystrophic mouse model of transient macrophage-depletion, mdxITGAM-DTR mice, in order to analyze the role of macrophage in skeletal muscle regeneration. We generated the transcriptome of satellite cells (SCs) and alpha7Sca1 cells purified by cell sorting from mdxITGAM-DTR mice. The mice were treated, by intramuscular injection, with PBS, as vehicle, or with Diphtheria toxin (DT) in order to achieve the macrophage depletion form hind-limb muscle We described a shift in identity of muscle stem cells dependent on the crosstalk between macrophages and satellite cells. Indeed macrophage depletion determines an exacerbated dystrophic phenotype associated with adipogenic conversion of SCs and reduction of the SC pool.

2019-09-17 | GSE134770 | GEO

ScRNA-seq of stromal and immune cells in WT and TGFBR2 KO mice

Project description:Single-cell RNA-seq in mice with subcutaneous tumors was used to determine if TGFβR2 signaling in DPT+ universal fibroblasts drives LRRC15+ myofibroblast differentiation using DptIresCreERT2Tgfbr2fl/fl and WT control mice.

2022-08-16 | E-MTAB-12028 | biostudies-arrayexpress

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [scRNA-seq]

Project description:To identify cellular heterogeneity and molecular signatures of bone marrow stromal (BMSCs) cells, we performed scRNA-seq of non-hematopoietic stromal cells from 3-week-old mice. In addition, to gain further insight into MSC differentiation in vivo, Pdgfrb-CreERT2 Rosa26-mTmG (Pdgfrb-Cre R26-mT/mG) double transgenic mice were treated with tamoxifen at P1-3 and sacrificed at P21 for the isolation of GFP+ cells from long bone followed by scRNA-seq analysis.

2021-07-27 | GSE156635 | GEO

scRNA-seq analysis of dendritic cells in skin draining lymph nodes of CLEC4C-DTR mice treated with FITC hapten

Project description:scRNA-seq analysis of dendritic cells in skin draining lymph nodes of CLEC4C-DTR mice treated with FITC hapten

| PRJNA678882 | ENA