Project description:Candida glabrata lab strain BG2 was used as parent to obtain some aneuploids by exposure to tunicamycin. Transcriptomes of 9 aneuploids bearing different chromosome change were compared to parent BG2
Project description:Several aneuploid Candida glabrata strains, each bearing a unique karyotype, were spread on YPD plates. They all exhibited colony size vairations. Randmly 10 large colonies from each strain were sequenced.
Project description:Candida albicans lab strain SC5314, which is a euploid diploid, was used to generate aneuploid fluconazole adaptors. Transcriptomes of aneuploids were compared to the euploid parent by growing cells in rich YPD broth.
Project description:We obtained ketoconazole tolerant mutants in Candida albicans after exposing the strain SC5314 to inhibitory concentration of ketoconazole. We did RNA-Seq to compare the transcriptomes of the aneuploid strains versus the parent euploid strain.
Project description:In Candida parapsilosis, transcriptomes of two aneuploid adaptors, TJ60 and TJ74, were compared to the euploid parent #12108. Cells were grown in YPD broth at 37C from OD600=0.1 to 1.0. Cells were collected and RNA was extracted.
Project description:In Candida glabrata, 8 euploid strains derived from BG2, were daily passaged in YPD broth for 10 times. Then the strains were sequenced.