Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression pattern of single mES cells during cell cycle stages

ABSTRACT: Gene expression pattern of single mES cells during cell cycle stages

PROVIDER: PRJEB6989 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR580012_1.fastq.gz	Fastqsanger.gz
	ERR580012_2.fastq.gz	Fastqsanger.gz
	ERR580013_1.fastq.gz	Fastqsanger.gz
	ERR580013_2.fastq.gz	Fastqsanger.gz
	ERR580014_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 576

Similar Datasets

Gene expression pattern of single mES cells during cell cycle stages

Project description:In this study, we aimed to study the gene expression patterns at single cell level across the different cell cycle stages in mESC. We performed single cell RNA-Seq experiment on mESC that were stained with Hoechst 33342 and Flow cytometry sorted for G1, S and G2M stages of cell cycle. Single cell RNA-Seq was performed using Fluidigm C1 system and libraries were generated using Nextera XT (Illumina) kit.

2014-12-29 | E-MTAB-2805 | biostudies-arrayexpress

Single cell gene expression patterns across different cell cycle stages in mouse embryonic cells

Project description:This study is aimed to decipher the gene expression patterns that exist across the different cell cycle stages at level of individual cells and their inherent heterogeneity. Conventional studies in yeast and human cells have shown coordination between gene expression and cell-cycle. However such a study for mouse ES cells (mES) and their differentiation has not been performed at a single cell level. In this study, we obtain pure mES cells from different cell cycle stages using FACS and apply single cell RNA-sequencing using the Fluidigm C1 system. This work will decipher and unravel the transcriptional patterns that coordinated with cell cycle progression at a single cell level.

2014-04-28 | E-ERAD-240 | biostudies-arrayexpress

mES phosphoproteome data - mES phosphoproteome data

Project description:mES phosphoproteome data

2010-12-17 | PRD000256 | Pride

mes-2, mes-4 or mes-2; mes-4 mutants vs. wild type

2012-05-30 | GSE38158 | GEO

Transcript and chromatin analysis in mes-2, mes-4, and mes-2; mes-4 double mutants

Project description:This SuperSeries is composed of the following subset Series: GSE38158: mes-2, mes-4 or mes-2; mes-4 mutants vs. wild type GSE38159: Strome MES-4, H3K36me3 and H3K27me3 in mes-4 RNAi EEMB GSE38180: Strome Mes-4, H3K36me3 and H3K27me3 in N2 EEMB Refer to individual Series

2012-05-29 | E-GEOD-38160 | biostudies-arrayexpress

ChIP of SMC2 at defined cell cycle stages

Project description:ChIP of SMC2 at defined cell cycle stages

| PRJNA510321 | ENA

Brugia malayi transcriptomics at different life cycle stages

Project description:Brugia malayi transcriptomics at different life cycle stages

| PRJEB2709 | ENA

mes-2, mes-4 or mes-2; mes-4 mutants vs. wild type

Project description:Trascriptional profiling of C. elegans adult germ lines comparing mes-2(bn11)unc-4(e120) mutants and unc-4(e120) (wild type), mes-4(bn85) mutants and N2 (wild type), mes-2(bn11)unc-4(e120); mes-4(bn85) and unc-4(e120) (wild type) at 20 degrees. One-condition experiment, mutant vs. WT. Biological replicates 4 mutant, 4 wildtype harvested indepedently. One mutant replicate and one WT replicate per array.

2012-05-29 | E-GEOD-38158 | biostudies-arrayexpress

C. elegans embryos: control vs. mes-2 mutant

Project description:Transcriptional profiling of early C. elegans embryos comparing control (N2) embryos with mes-2 mutant embryos at different developmental stages: 2E (24-40 cells), 4E (50-90 cells) and 8E stage (100-200 cells). Goal was to determine the effects of mes-2 loss on global gene expression as embryos transit from a developmentally plastic state (2E stage) to the onset of differentiation (8E stage). Our microarray data showed that early-expressed genes remain active, differentiation genes fail to reach wild-type levels in mes-2 mutant embryos at the 8E stage.

2009-05-26 | GSE14913 | GEO

C. elegans embryos: control vs. mes-2 mutant

2010-05-18 | E-GEOD-14913 | biostudies-arrayexpress