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IL10 Chicken Project

ABSTRACT: 16S analysis of caecal contents of IL10 chicken

PROVIDER: PRJEB71960 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR12512789_1.fastq.gz	Fastqsanger.gz
	ERR12512789_2.fastq.gz	Fastqsanger.gz
	ERR12512790_1.fastq.gz	Fastqsanger.gz
	ERR12512790_2.fastq.gz	Fastqsanger.gz
	ERR12512791_1.fastq.gz	Fastqsanger.gz

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Project description:Campylobacter jejuni is the major cause of acute gastroenteritis in the developed world. It is usually acquired through contaminated poultry as C. jejuni causes a silent asymptomatic infection of the chicken. Pathogens face different sources of stress during its transit through the gut. In this study, we describe the ability of C. jejuni to survive nitrosative stress at very low oxygen levels that reflect those in hypoxic gut environments. Specifically, we here explore an innovative model of signal recognition during colonization. We use a diffusion capsule to feed small, diffusible molecules from chicken caecal matter into a microaerobic C. jejuni culture to study the transcriptomic changes mounted as response to chemical signals present in the chicken gut. We find that in early stages of exposure to the caecal contents (10 min) the dual component colonization regulator, dccR, plays an important yet not fully understood role. Although the caecal material contains cyanide derived from plant sources, we find no role for a truncated globin (encoded by ctb), which has previously been implicated in resistance to this haem ligand.

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