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XpLepCine

ABSTRACT: Lepidochitona cinerea (grey chiton)

PROVIDER: PRJEB72111 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	ERR12535451.fastq.gz	Fastqsanger.gz
	ERR12535452.fastq.gz	Fastqsanger.gz
	ERR12542988_1.fastq.gz	Fastqsanger.gz
	ERR12542988_2.fastq.gz	Fastqsanger.gz

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1 - 5 of 17

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PPH24 - Botrytis cinerea Steroidal glycoalkaloids

Project description:Multiple mechanisms of the grey mould Botrytis cinerea to detoxify plant antifungal steroidal glycoalkaloids

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Increased protein solubility contributes to heat priming of the plant pathogenic fungus Botrytis cinerea

Project description:Botrytis cinerea causes grey mold disease in leading crop plants. The disease develops only at cool temperatures, but the fungus remains viable in warm climates and can survive periods of extreme heat. We discovered a strong heat priming effect in which the exposure of B. cinerea to moderately high temperatures greatly improves its ability to cope with subsequent, potentially lethal temperature conditions. We showed that priming promotes protein solubility during heat stress and discovered a group of priming-induced serine-type peptidases. Several lines of evidence, including transcriptomics, proteomics, pharmacology and mutagenesis data, link these peptidases to the B. cinerea priming response, highlighting their important roles in regulating priming-mediated heat adaptation.

2023-06-18 | PXD037173 | Pride

Comparative proteomic analysis of the exoproteome of a wild type and cps1 mutant strains of the filamentous fungus Botrytis cinerea.

Project description:Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide, including economically important crops. Considering the central role of the fungal cell wall in the interaction between fungi and plants, the role of a conserved putative polysaccharide synthase in the physiology, development and virulence of B. cinerea was explored. To this aim, the BcCps1 gene was deleted and the mutant strain was characterized. In order to reveal the impact of the mutation on the fungal secretion, the exo-proteome of the wild type and mutant strains were prepared for comparative analysis of their contents.

2022-10-15 | PXD033590 | Pride

Proteomic analysis of the extracellular vesicles of the filamentous fungus Botrytis cinerea.

Project description:Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide. Considering the central role of secretion in the necrotrophic interaction between the fungus and its host plants, non conventional secre-tion was explored through the search and characterisation of extracellular vesicles. In or-der to reveal the protein composition of these vesicles, a proteomic analysis was chosen.

2023-05-02 | PXD035766 | Pride

Increased protein solubility contributes to heat priming of the plant pathogenic fungus Botrytis cinerea

2023-06-18 | GSE216541 | GEO

Whole-transcriptome microarray analysis of grapevine-Botrytis cinerea interaction during latent infection of berries (“noble rot”)

Project description:Purpose: Microarray technologies provide a unique opportunity to deeply investigate the molecular mechanisms involved in plant-pathogen interaction. Botrytis cinerea, is the agent of grapevine grey mould, but in yet uncharacterized environmental conditions, a latent infection can occur determining favourable metabolic and physico-chemical berry modifications which possibly contribute to the typical aromas of “passito” wines (“noble rot”). The present project aims at the identification of the grapevine responses to B. cinerea during fungal colonization in the latent form, in comparison with control berries. Methods: A total of 150 untreated berries were sampled as time 0 of the experiment. Moreover, 300 healthy berries have been artificially inoculated one by one with B. cinerea by injecting conidia under berry skin, in controlled conditions, reproducing an early stage (T1) and a late stage (T2) of noble rot pourri plein. Control samples (300 berries) have been inoculated with water and sampled at the same time of infected berries. The microarray experiments on T0 and healthy or infected samples in biological triplicate resulted in 15 samples to be analyzed (Agilent-048771 4x44K Grape all custom microarray chip; Agilent Technologies, Santa Clara, CA, USA). Conclusions: This work identified important molecular mechanisms involved in Botrytis cinerea colonization of grapevine berries during the noble rot infection.

2019-07-26 | GSE116740 | GEO

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