Project description:Here we investigate the function of CUC1(CUP-SHAPED COTYLEDON1) in the diversification of leaf forms between simple-leaved Arabidopsis thaliana and compound-leaved Cardamine hirsuta. CUC transcription factors are conserved regulators in leaf margin dissection and leaflet formation. ChCUC1, ChCUC2 and ChCUC3 function redundantly and are required for the leaflet formation in C. hirsuta. Recently we discovered that ChCUC1 has species species-specific expression in young leaves of C.hirsuta. Moreover, interspecies gene transfer of ChCUC1 allele into A.thaliana is sufficient to increase leaf complexity. On this basis, we hypothesize that redeployment of ChCUC1 in leaves contributes to the formation of leaflets instead of serrations. However, the mechanism underlying ChCUC1 regulating cell division, cell polarity, cytoskeleton and thus leaf marginal patterning remains elusive. To this end, we make use of chromatin immunoprecipitation sequencing(ChIP-seq), transcriptomic, comparative genetics and advanced imaging approaches to identify the downstream regulating genes of ChCUC1.
Project description:Transcriptomic profile of Arabis alpina apex enriched material. We compared apices from plants grown for 2 weeks in long days (20M-BM-0C) (juvenile) with apices from plants grown for 8 weeks in long days (20M-BM-0C) (adult) and apices from plants grown for 2 weeks and then shifted in vernalization for 4 weeks with apices from plants grown for 8 weeks and than shifted to vernalization for 4 weeks. 2x2 factorial design. 2 starting ages (2 weeks and 8 weeks) and 2 developmental conditions (before and after vernalization). 4 biological replicates for each condition (16 arrays). Reference: pool of the 16 arrays.
Project description:Transcriptomic profile of Arabis alpina apex enriched material. We compared apices from plants grown for 2 weeks in long days (20°C) (juvenile) with apices from plants grown for 8 weeks in long days (20°C) (adult) and apices from plants grown for 2 weeks and then shifted in vernalization for 4 weeks with apices from plants grown for 8 weeks and than shifted to vernalization for 4 weeks.
2013-05-27 | GSE40455 | GEO
Project description:DNA sequencing data of Uncaria hirsuta
