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Single cell transcriptomic analysis of mutant MECP2 human neural cells over a time-course of in vitro differentiation

ABSTRACT: Single cell transcriptomic analysis of mutant MECP2 human neural cells over a time-course of in vitro differentiation

PROVIDER: PRJEB75396 | ENA |

REPOSITORIES: ENA

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Single cell transcriptomic analysis of mutant MECP2 human neural cells over a time-course of in vitro differentiation

Project description:Rett Syndrome (RTT) is a severe neurological disorder predominantly affecting females, caused by mutations in the methyl CpG binding protein 2 (MECP2) gene. Understanding the pathophysiology of RTT at a cellular and molecular level is crucial for the development of targeted therapies. Our project aims to dissect the molecular underpinnings of RTT using a novel in vitro model system based on a commercially available human neural progenitor cell line, ReNCell. We have engineered multiple distinct ReNCell lines to mimic specific genetic alterations associated with RTT, providing a robust platform for mechanistic studies and drug screening. This cell line is a complete knockout of MECP2, serving as a model to investigate the effects of total loss of MeCP2 function. This model helps in understanding the full spectrum of MeCP2's roles in neural development and maintenance, and in identifying compensatory mechanisms that could be targeted therapeutically. We capture the progenitor state (0 days), and differentiation states at 3, 7, 14, 21 and 30 days for both the MECP2 knockut and the corresponding wildtype.

2024-11-17 | E-MTAB-14047 | biostudies-arrayexpress

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2024-11-17 | E-MTAB-14046 | biostudies-arrayexpress

Over-expression of either MECP2_e1 or MECP2_e2 in mouse MECP2 knockout fibroblasts

Project description:Aim: to detect genes that are differentially transcribed in mouse MECP2 knockout fibroblasts over-expressing either of the two human MECP2 isoforms, MECP2_e1 or MECP2_e2. Methods: mouse Mecp2tm1.1Bird knockout fibroblast cell line was stably infected by lentiviral vectors over-expressing MECP2_e1, MECP2_e2, or eGFP. RNA extracted, and used for gene expression microarray analysis

2014-01-25 | GSE54261 | GEO

Transcriptomic analysis of mutant MECP2 and NEAT1 human neural cells

Project description:Rett Syndrome (RTT) is a severe neurological disorder predominantly affecting females, caused by mutations in the methyl CpG binding protein 2 (MECP2) gene. Understanding the pathophysiology of RTT at a cellular and molecular level is crucial for the development of targeted therapies. This project aims to dissect the molecular underpinnings of RTT using a novel in vitro model system based on a commercially available human neural progenitor cell line, ReNCell. We have engineered multiple distinct ReNCell lines to mimic specific genetic alterations associated with RTT, providing a robust platform for mechanistic studies and drug screening. One cell line is a complete knockout of MECP2, serving as a model to investigate the effects of total loss of MeCP2 function. This model helps in understanding the full spectrum of MeCP2's roles in neural development and maintenance, and in identifying compensatory mechanisms that could be targeted therapeutically. The other line involves the knockdown of NEAT1, a long non-coding RNA known to be involved in the pathogenesis of several neurological disorders, including RTT. Recent studies suggest NEAT1 plays a critical role in the neuronal cellular response to MECP2 dysfunction. By reducing NEAT1 expression, we aim to elucidate its contribution to RTT pathology and explore its potential as a therapeutic target. Here we characterize the transcriptome of these cell lines, including the wild type (control), at the progenitor state and after 7 days of differentiation with three replicates each.

2024-11-17 | E-MTAB-14048 | biostudies-arrayexpress

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Over-expression of either MECP2_e1 or MECP2_e2 in mouse MECP2 knockout fibroblasts

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Global gene expression analysis of OSKM / N2SKM- infected MEFs over time course

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2010-01-22 | GSE19022 | GEO

Transcriptomic analysis of mutant MECP2 human neural progenitor cells

Project description:Transcriptomic analysis of mutant MECP2 human neural progenitor cells

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Circadian time course

Project description:Circadian time course

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RNA expression analysis of cells producing the synthetic protein DX over a 4hr time course

Project description:We expressed the man-made protein DX in E. coli cells over the course of 4 hours and examined effects on global gene expression.

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