Project description:ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data with transcriptomic data obtained by microarrays. Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)
Project description:A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS
Project description:We purified two populations of human ileal enteroendocrine cells (Venus+ and Venus-) from mature differentiated hGLU-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages.
Project description:We purified two populations of human duodenal MLN-expressing enteroendocrine cells (Venus+ and Venus-) from mature differentiated hMLN-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages.
